FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate4_12.3520000005d6a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate4_12.3520000005d6a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39425
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4391.1135066582117945No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA4351.1033608116677236No Hit
GCGTAAGGATTCACGTACTCTGCGTTGATACCA4071.0323398858592263No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA3500.8877615726062142No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA2850.7228915662650602No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA2680.6797717184527584No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA2560.6493341788205453No Hit
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA2400.6087507926442612No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA2330.590995561192137No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA2120.5377298668357641No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA2040.517438173747622No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA1990.5047558655675333No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA1960.4971464806594801No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA1920.487000634115409No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA1640.41597970830691183No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA1570.39822447685478757No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC1460.3703233988585923No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA1170.2967660114140774No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA1050.2663284717818643No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA970.24603677869372226No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGATAA930.23589093214965123No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA900.22828154724159797No Hit
GAGTGAATGGGGCGTAAGGAAAAAAAAAAAAAA890.2257450856055802No Hit
ACGCAGAGTGAATGGGGCGTAAGGATAAAAAAA830.21052631578947367No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.20291693088142043No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGAAAA790.20038046924540265No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.20038046924540265No Hit
AACGCAGAGTGAATGGGCGTAAGGAAAAAAAAA770.19530754597336716No Hit
GGCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAA750.19023462270133165No Hit
GAATGGGCGTAAGGAAAAAAAAAAAAAAAAAAA750.19023462270133165No Hit
GTATCAACGCAGAGTGAATGGGATTCACGTACT730.18516169942929614No Hit
ACGCAGAGTGAATGGGGCGTAAGGAAAAAAAAA730.18516169942929614No Hit
GAATGGGCGTAAGGATAAAAAAAAAAAAAAAAA720.18262523779327838No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.17755231452124287No Hit
TATCTACGCAGAGTGAATGGGCGTAAGGATAAA700.17755231452124287No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA670.1699429296131896No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA660.16740646797717185No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATTCA640.16233354470513633No Hit
GAGTGAATGGGATTCACGTACTCTGCGAAAAAA630.15979708306911858No Hit
GAATGGGGCGTAAGGATAAAAAAAAAAAAAAAA630.15979708306911858No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA620.15726062143310082No Hit
GTGAATGGGAGCGTAAGGATAAAAAAAAAAAAA610.15472415979708307No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC580.1471147748890298No Hit
ATCAACGCAGAGTGAATGGGCGTAAGGATAAAA580.1471147748890298No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGATAA580.1471147748890298No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA570.14457831325301204No Hit
AACGCAGAGTGAATGGGGCGTAAGGATAAAAAA570.14457831325301204No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGAAAA570.14457831325301204No Hit
ACGCAGAGTGAATGGGCGTAAGGATTCACGTAC560.14204185161699429No Hit
GAATGGGGCGTAAGGAAAAAAAAAAAAAAAAAA510.1293595434369055No Hit
GTGAATGGGGGCGTAAGGAAAAAAAAAAAAAAA510.1293595434369055No Hit
GTGAATGGGGGCGTAAGGATAAAAAAAAAAAAA500.12682308180088775No Hit
GGCGTAAGGATTCACGTACTCTGCGTTGATACC500.12682308180088775No Hit
TATCTACGCAGAGTGAATGGGCGTAAGGAAAAA490.12428662016487002No Hit
GTATCATCGCAGAGTGAATGGGCGTAAGGATAA480.12175015852885225No Hit
CGTAAGGATAAAAAAAAAAAAAAAAAAAAAAAA480.12175015852885225No Hit
GTATCATCGCAGAGTGAATGGGCGTAAGGAAAA470.1192136968928345No Hit
CACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA460.11667723525681672No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA450.11414077362079898No Hit
GTGAATGGGCGTAAGGATTCACGTACTCAAAAA440.11160431198478124No Hit
CAACGCAGAGTGAATGGGCGTAAGGAAAAAAAA430.10906785034876347No Hit
GCGTAAGGATTCACGTACTCAAAAAAAAAAAAA430.10906785034876347No Hit
AACGCAGAGTGAATGGGATTCACGTACTCTGCG420.10653138871274573No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.10399492707672796No Hit
CAACTCAGAGTGAATGGGCGTAAGGATAAAAAA410.10399492707672796No Hit
ATCAACGCAGAGTGAATGGGCGTAAGGAAAAAA410.10399492707672796No Hit
CAACGCAGAGTGAATGGGCGTAAGGATAAAAAA410.10399492707672796No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGA400.10145846544071022No Hit
GTATCAACGCAGAGTGAATGGGGCGTAAGGAAA400.10145846544071022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT306.9401205E-527.0267184
TAGAGTG306.9401205E-527.0267185
ACGTAGA307.000678E-526.9923762
CGTAGAG307.000678E-526.9923763
GTATCAT405.913116E-726.9581221
AACGTAG307.061684E-526.958121
CAACTCA501.5081241E-724.262311
TATCATC504.0795385E-621.59392
AACTCAG608.631414E-720.2442822
TCGCAGA559.206224E-619.6557947
ATCGCAG559.206224E-619.6557946
TCATCGC559.206224E-619.6557944
CATCGCA559.206224E-619.6557945
GTATCAA1450.019.5213991
CTCAGAG651.8365099E-618.7108044
TCAGAGT601.9495365E-518.0178135
CACGCGT450.001077333718.017816
CTACGCA450.001077333718.017814
ATCTACG450.001086600917.9949172
TCTACGC450.001086600917.9949173
Produced by FastQC (version 0.11.2)