FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_94.3520000005d4d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_94.3520000005d4d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81135
Sequences flagged as poor quality0
Sequence length33
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA2170.2674554754421643No Hit
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC2070.25513033832501386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.24034017378443337No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA1600.19720219387440685No Hit
CCCCCCCTCCTTGCAGTAACGTACTCTGCGTTG1000.12325137117150428No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC970.11955383003635915No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA840.1035311517840636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT455.470247E-827.0173071
TATCATC307.0768176E-527.0006182
GTAGAGT200.00856304327.0006164
CTATACC200.00856304327.0006163
ACGTAGA200.00856304327.0006162
CGTAGAG200.00856304327.0006163
TAGAGTG200.00856304327.0006165
AACTCAG501.5264959E-724.3005562
TCAGAGT451.6650501E-624.000555
CTCAGAG451.6650501E-624.000554
TATCTAC603.5157427E-822.5144211
CAACTCA553.8066173E-722.105071
ACTCAGA553.829391E-722.0914153
TGCGTTG8050.020.12468327
ATCTACG559.485935E-619.6368142
TCTACGC559.485935E-619.6368143
CTACGCA559.485935E-619.6368144
TAACTTA350.005488747719.28615622
GTAACTT350.005488747719.28615621
AGGCTCC350.00550839919.2742526
Produced by FastQC (version 0.11.2)