FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_93.3520000005d4b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_93.3520000005d4b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23166
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT600.25900025900025897No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA590.25468358801692137No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA510.22015022015022012No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA450.19425019425019424No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA380.1640334973668307No Hit
GTGAATGGGGTAGCGTCAAAAAAAAAAAAAAAA370.15971682638349305No Hit
GTGAATGGGATAGCGTCAAAAAAAAAAAAAAAA350.15108348441681776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1381334714668048No Hit
GTGAATGGGTAGCGTCGAGATAAAAAAAAAAAA320.1381334714668048No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA300.12950012950012948No Hit
GTGAATGGGTAGCGTCGAAAAAAAAAAAAAAAA290.12518345851679186No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA280.12086678753345419No Hit
TATCAACGCAGAGTGAATGGGATAGCGTCGAGA280.12086678753345419No Hit
GTGAATGGGATAGCGTCGAGAAAAAAAAAAAAA270.11655011655011654No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA270.11655011655011654No Hit
GTGAATGGGTAGCGTCAAAAAAAAAAAAAAAAA270.11655011655011654No Hit
GTGAATGGGGTAGCGTAAAAAAAAAAAAAAAAA270.11655011655011654No Hit
TATCAACGCAGAGTGAATGGGTAGCGTCGAGAT270.11655011655011654No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA250.10791677458344125No Hit
GTGAATGGGGTATCTCGACGCTAAAAAAAAAAA250.10791677458344125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1500.018.91
TCAACGC1700.016.6764723
ATCAACG1700.016.6764722
CAACGCA2000.014.854
TGCGTTG1900.014.21052627
TCTGCGT4850.013.63917527
AACGCAG2200.013.55
ACGCAGA2750.011.7818176
CTGCGTT2356.002665E-1110.91489326
CGCAGAG2850.010.8947387
GCAGAGT2653.6379788E-1210.6981138
CAGAGTG2705.456968E-1210.59
AATGGGG1701.2443979E-610.3235294
AGAGTGA2759.094947E-1210.30909110
CTCTGCG6500.010.17692326
GTATCAA1205.7287916E-410.1251
ACTCTGC6900.09.58695625
TACTCTG6900.09.58695624
CGTACTC7000.09.45000122
ACGTACT7150.09.44055821
Produced by FastQC (version 0.11.2)