FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_9.3520000005ca4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_9.3520000005ca4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10981
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC1211.101903287496585No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC510.4644385757217011No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA490.4462252982424187No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA480.4371186595027775No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC450.4097987432838539No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA430.3915854658045715No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA300.27319916218923596No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA250.22766596849103No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA250.22766596849103No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC250.22766596849103No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA240.21855932975138875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.19123941353246515No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC180.16391949731354155No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA170.15481285857390037No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA160.1457062198342592No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA150.13659958109461798No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA140.12749294235497677No Hit
TCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA130.11838630361533557No Hit
ACGCAGAGTGAATGGGCTACGTACCGAAAAAAA120.10927966487569438No Hit
CTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA120.10927966487569438No Hit
GCTACGTACAAAAAAAAAAAAAAAAAAAAAAAA110.1001730261360532No Hit
GCTACGTACCAAAAAAAAAAAAAAAAAAAAAAA110.1001730261360532No Hit
ACTACGTACCGTAAAAAAAAAAAAAAAAAAAAA110.1001730261360532No Hit
CCGTAACGTACTCAAAAAAAAAAAAAAAAAAAA110.1001730261360532No Hit
GAGTGAATGGGCTACGTACCGAAAAAAAAAAAA110.1001730261360532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC404.6024667E-420.2527
TTGATAC500.002061599616.19999926
TCTGCGT2450.012.1224527
GTGAATG1802.4011035E-59.01
GAATGGG1853.3194516E-58.7567573
CTCTGCG3403.6379788E-118.73529326
TGCGTTG1250.0086099248.6427
TGAATGG1751.8281114E-48.4857142
ACTCTGC3803.6743586E-107.815789725
TACTCTG4202.8412614E-97.071428324
ACGTACT4304.5765773E-96.906976721
CGTACTC4304.5765773E-96.906976722
GTACTCT4355.7825673E-96.82758623
AACGTAC4407.2850526E-96.7520
TAACGTA4501.1457814E-86.619
CCGTAAC4551.4306352E-86.527472516
CGTAACG4551.4306352E-86.527472517
GTAACGT4551.4306352E-86.527472518
GTACCGT4753.3849574E-86.25263113
TACCGTA4753.3849574E-86.25263114
Produced by FastQC (version 0.11.2)