FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_89.3520000005d44e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_89.3520000005d44e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA40.3703703703703704No Hit
GAATGGGCCTATCGGTAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
CCTATCGGTTGAACGTACTCTGCGTTGATACCA30.2777777777777778No Hit
ACCTATCGGTTGAACGTACTCTGCGTTGATACC30.2777777777777778No Hit
GTGAATGGGGCCTATCGGAAAAAAAAAAAAAAA30.2777777777777778No Hit
GCAGAGTACGTTCAAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
TATCAACGCAGAGTGAATGGGCCTATCGGAAAA20.1851851851851852No Hit
GTGAATGGGACGTCCTATCGGTAAAAAAAAAAA20.1851851851851852No Hit
GGGCTGCCTATCGGAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GCCTCCTATCGGTTGAACGTACTCTGCGTTGAT20.1851851851851852No Hit
GAATGGGACCTATCGGAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GGACTACCTATCGGTAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
TGGTACGACCTATCGGTTGAACGTACTCTGCGT20.1851851851851852No Hit
GTACGTTCAACCAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GAGTGAATGGGCCTATCGGTTGAACGTACTCTG20.1851851851851852No Hit
ATCAACGCAGAGTGAATGGGATAGGCAAAAAAA20.1851851851851852No Hit
GCCCTATCGGTAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GAATGGGACCTATCGAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CCTCGATCCCTATCGGTTGAACGTACTCTGCGT20.1851851851851852No Hit
ATTTATTATCCTATCGGTTGAACGTACTCTGCG20.1851851851851852No Hit
GCCTATCGGAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGGCGTCCTAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTTTTCCACCTATCGGTTGAACGTACTCTGCGT20.1851851851851852No Hit
ACGCAGAGTGAATGGGGCCGCCAAAAAAAAAAA20.1851851851851852No Hit
CTGGGTGGCCTATCGGTTGAACGTACTCTGCGT20.1851851851851852No Hit
GTTAGAGCCCTATCGGTTGAACGTACTCTGCGT20.1851851851851852No Hit
CCTCCCCTATCGGTTGAACGTACTCTGCGTTGA20.1851851851851852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT451.1171205E-521.027
CTCTGCG601.0487745E-415.75000126
AACGTAC601.0487745E-415.75000120
GTTGAAC601.0487745E-415.75000116
GGTTGAA601.0487745E-415.75000115
TCGGTTG601.0487745E-415.75000113
GTACTCT601.0487745E-415.75000123
GAACGTA601.0487745E-415.75000119
TATCGGT601.0487745E-415.75000111
ACGTACT601.0487745E-415.75000121
ACTCTGC601.0487745E-415.75000125
ATCGGTT601.0487745E-415.75000112
CGTACTC601.0487745E-415.75000122
TTGAACG601.0487745E-415.75000117
CGGTTGA601.0487745E-415.75000114
TGAACGT601.0487745E-415.75000118
TACTCTG601.0487745E-415.75000124
CCTATCG703.4533843E-413.59
CTATCGG703.4533843E-413.510
Produced by FastQC (version 0.11.2)