FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_87.3520000005d40a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_87.3520000005d40a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37569
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC1250.3327211264606457No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1140.3034416673321089No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT720.19164736884133196No Hit
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA630.16769144773616546No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA570.15172083366605446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.1490590646543693No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC500.1330884505842583No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT490.13042668157257314No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA440.1171178365141473No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG440.1171178365141473No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA440.1171178365141473No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT430.11445606750246215No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA420.11179429849077697No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA380.1011472224440363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTATA200.00850644527.02
AGCCAGG351.9883561E-423.14285926
TCTACGA351.9883561E-423.1428592
GCCAGGC351.9883561E-423.14285927
CGAGTCG351.9883561E-423.1428596
GTCTACG351.9883561E-423.1428591
CTACGAG351.9883561E-423.1428593
GCAGCCC300.002224318522.527
GGGTTGT404.9039366E-420.2512
CCTATAC404.9039366E-420.252
CTATACC404.9039366E-420.253
GGGCAAG404.9039366E-420.2519
TATACCC404.9039366E-420.254
CGGGGCA404.9039366E-420.2517
GGCAAGA404.9039366E-420.2520
GAGCCAG404.9039366E-420.2525
CGGGTTG404.9039366E-420.2511
ACGAGTC404.9039366E-420.255
AGAGCCA404.9039366E-420.2524
TACGAGT404.9039366E-420.254
Produced by FastQC (version 0.11.2)