FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_86.3520000005d3ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_86.3520000005d3ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42010
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCAACAACGTACT3950.940252320875982No Hit
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA2150.5117829088312307No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC1650.39276362770768863No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA1530.3641990002380386No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC1440.342775529635801No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA1150.27374434658414665No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA1130.26898357533920497No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA1120.2666031897167341No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC1100.26184241847179246No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA1050.24994049035943822No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA1010.24041894786955487No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC920.21899547726731727No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC850.2023327779100214No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA810.19281123542013806No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA770.1832896929302547No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA760.18090930730778387No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG720.1713877648179005No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA700.16662699357295882No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC630.14996429421566293No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA630.14996429421566293No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA620.1475839085931921No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA600.14282313734825042No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA590.14044275172577958No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA580.13806236610330874No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA580.13806236610330874No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA560.13330159485836704No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA550.13092120923589623No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTAAA540.12854082361342536No Hit
GTGAATGGGTGTTGCCAAAAAAAAAAAAAAAAA530.12616043799095453No Hit
CAACAACGTACTCTGCGAAAAAAAAAAAAAAAA520.12378005236848369No Hit
GTGAATGGGGTTGTTGCCAAAAAAAAAAAAAAA500.119019281123542No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA500.119019281123542No Hit
GTGAATGGGCAACAACGTACTCTAAAAAAAAAA450.10711735301118781No Hit
GTGAATGGGCAACAACGTACTCTGCGAAAAAAA450.10711735301118781No Hit
GTGTTCGGCAACAACGTACTCTGCGTTGATACC440.10473696738871696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCCC200.00851779927.027
TCGCAGA200.00851779927.07
ATCGCAG200.00851779927.06
TCATCGC200.00851779927.04
CATCGCA200.00851779927.05
TCGGGTT401.7990786E-523.62510
GAGTCGG351.9938641E-423.1428597
CGGGTTG351.9938641E-423.14285911
ACGAGTC351.9938641E-423.1428595
AGTCGGG351.9938641E-423.1428598
TACGAGT351.9938641E-423.1428594
CGAGTCG351.9938641E-423.1428596
CTACGAG351.9938641E-423.1428593
GTCGGGT351.9938641E-423.1428599
TCTACGA404.9173704E-420.252
GTCTACG404.9173704E-420.251
ATGGGGA350.00544630819.2857155
TTGGGAA350.00544630819.28571519
TATCAAC1600.017.718751
ATCAACG1750.016.1999992
Produced by FastQC (version 0.11.2)