FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_85.3520000005d3c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_85.3520000005d3c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32406
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA4091.262111954576313No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.6480281429364932No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA1840.567796087144356No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA1660.5122508177497994No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC1200.37030179596371043No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA1200.37030179596371043No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA1160.3579584027649201No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA1030.3178423748688515No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA950.2931555884712707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.2561254088748997No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.24069616737641178No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA640.19749429118064557No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA580.17897920138246004No Hit
TATCAACGCAGAGTGAATGGGTACTAGAGACGT520.1604641115842745No Hit
TATCAACGCAGAGTGAATGGGTCGGTACTAGAG390.12034808368820589No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA370.11417638708881071No Hit
TATCAACGCAGAGTGAATGGGTCGGTACAAAAA360.11109053878911312No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC360.11109053878911312No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA360.11109053878911312No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA360.11109053878911312No Hit
GTGAATGGGGTCGGTACAAAAAAAAAAAAAAAA350.10800469048941554No Hit
TATCAACGCAGAGTGAATGGGATCGGTACTAGA340.10491884218971795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA504.833055E-927.0123841
ATCTACG504.833055E-927.0123842
GTATCAT800.027.0123841
TATCTAC504.833055E-927.0123841
CTACGCA504.833055E-927.0123844
CCCCTCG200.00847026527.0123845
CCGACCA200.00853460826.9706349
TATCATC850.025.4234222
ATCATCG850.025.4234223
TCATCGC850.025.4234224
CATCGCA850.025.4234225
TCGCAGA751.8189894E-1225.1725927
ATCGCAG751.8189894E-1225.1725926
TCTACGC551.3335011E-824.5567133
TACGCAG551.3335011E-824.5567135
AACTCAG653.081368E-922.8566342
CACTCGG553.7237623E-722.0668816
TCTGCGT5150.021.80100827
ACTCAGA707.2523108E-921.2240163
CTCAGAG707.2523108E-921.2240164
Produced by FastQC (version 0.11.2)