FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_81.3520000005d34f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_81.3520000005d34f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19024
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA780.4100084104289319No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA770.4047518923465097No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT770.4047518923465097No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA670.35218671152228764No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA650.3416736753574432No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA640.33641715727502103No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA630.3311606391925988No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA590.31013456686291No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA490.25756938603868795No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA480.2523128679562658No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.2470563498738436No Hit
GCCATTCCGTTACGTACTCTGCGTTGATACCAC430.22603027754415475No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC410.21551724137931033No Hit
TATCAACGCAGAGTGAATGGGGAGTACGTAACG380.19974768713204374No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA380.19974768713204374No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA370.19449116904962152No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT360.18923465096719932No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA350.18397813288477713No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT320.16820857863751051No Hit
GTGAATGGGGAGTACGTAACGGAAAAAAAAAAA270.14192598822539948No Hit
GTACGTAACGGAAAAAAAAAAAAAAAAAAAAAA260.13666947014297728No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA260.13666947014297728No Hit
CCCCCAGCCATTCCGTTACGTACTCTGCGTTGA260.13666947014297728No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.13666947014297728No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC260.13666947014297728No Hit
GTGAATGGGAGTACGTAACGAAAAAAAAAAAAA240.1261564339781329No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1261564339781329No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.1208999158957107No Hit
ACGCAGAGTGAATGGGGAGTACGTAACGAAAAA230.1208999158957107No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA220.11564339781328847No Hit
GTGAATGGGGTACGTAACGAAAAAAAAAAAAAA220.11564339781328847No Hit
GTGAATGGGGTACGTAACGGAAAAAAAAAAAAA200.10513036164844407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA1600.022.7812527
TATCAAC1950.019.3846151
TCAACGC2200.017.1818183
ATCAACG2250.016.8000012
CAACGCA2500.015.124
AACGCAG2700.014.55
TGCGTTG3450.014.47826127
GAGCCAA700.001256876513.525
ACGCAGA3550.011.4084516
CTGCGTT4400.011.35227226
CAGAGTG3000.011.2499999
AGAGTGA3050.011.06557410
CGCAGAG3550.011.0281697
GCAGAGT3250.010.7999998
GAGTGAA3650.09.24657511
AGTGAAT3650.09.24657512
AATGGGG2009.569312E-68.77516
TCTGCGT6250.08.20827
AAGCCAT1904.940288E-47.81578976
AGCCAAA1750.00194892157.714285426
Produced by FastQC (version 0.11.2)