FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_80.3520000005d318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_80.3520000005d318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37747
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC5561.4729647389196492No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA4591.2159906747556097No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA3971.0517392110631307No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA2760.7311839351471641No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA2660.704691763583861No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA2500.6623042890825761No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.601372294486979No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA2150.5695816886110154No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA1910.5060004768590882No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA1870.4954036082337669No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA1870.4954036082337669No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA1790.4742098709831245No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA1710.45301613373248206No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT1670.44241926510716084No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.42387474501284866No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA1480.392084139136885No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1370.36294275041725166No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA1320.3496966646356002No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA1280.33909979601027895No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA1270.33645057885394863No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.33115214454128805No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA1240.3285029273849578No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.3099584072906456No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC1100.2914138871963335No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC1090.28876467004000317No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC990.26227249847670014No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA950.2516756298513789No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA910.2410787612260577No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA880.23313110975706677No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA870.23048189260073648No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA860.22783267544440616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.22253424113174558No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA780.20663893819376375No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA780.20663893819376375No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA780.20663893819376375No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA770.20398972103743343No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG750.19869128672477285No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC730.19339285241211224No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA730.19339285241211224No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA640.16954989800513948No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC630.16690068084880919No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC590.15630381222348796No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA590.15630381222348796No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA580.15365459506715765No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA580.15365459506715765No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA580.15365459506715765No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA570.15100537791082735No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA570.15100537791082735No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.15100537791082735No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA560.14835616075449704No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA550.14570694359816674No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA540.14305772644183642No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA540.14305772644183642No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA540.14305772644183642No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA530.14040850928550613No Hit
GTATCATCGCAGAGTGAATGGGGCTAACTATCC520.13775929212917584No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA510.13511007497284552No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA510.13511007497284552No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA470.1245132063475243No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA460.121863989191194No Hit
GGGCTAACTATCCACGTACTCAAAAAAAAAAAA450.1192147720348637No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA450.1192147720348637No Hit
GAATGGGGCTAACTATCCACGTACTCAAAAAAA450.1192147720348637No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA440.11656555487853339No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA440.11656555487853339No Hit
GTGAATGGGGCTAACTATAAAAAAAAAAAAAAA430.11391633772220308No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA430.11391633772220308No Hit
TATCAACGCAGAGTGAATGGGCTAACTATAAAA430.11391633772220308No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA430.11391633772220308No Hit
TATCAACGCAGAGTGAATGGGGCTAACTAAAAA420.11126712056587279No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA390.10331946909688187No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA380.10067025194055157No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA380.10067025194055157No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA380.10067025194055157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCT200.00850701227.06
GTATCAT554.5292836E-1026.9999981
TATCATC554.5292836E-1026.9999982
TCGCAGA751.6356353E-819.87
ATCATCG751.6356353E-819.83
ATCGCAG751.6356353E-819.86
TCATCGC751.6356353E-819.84
TCCCGGC350.00543624319.2857155
CATCGCA803.4313416E-818.56255
GATACCA500.00219385216.19999927
TGCGTTG2450.015.97959227
ACGGCTA1104.491085E-915.9545447
TATCAAC2700.015.01
TCTGCGT8050.014.25465827
TGATACC600.007374565613.50000126
ATCGGCT600.007374565613.5000016
GTATCAA1501.2951205E-913.4999991
TCAACGC3200.012.656253
GGTATCA750.002182441512.5999991
ATCAACG3150.012.4285722
Produced by FastQC (version 0.11.2)