FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_8.3520000005ca21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_8.3520000005ca21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA292.843137254901961No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA111.0784313725490196No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.8823529411764706No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA90.8823529411764706No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA80.7843137254901961No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC80.7843137254901961No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA70.6862745098039216No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA70.6862745098039216No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA60.5882352941176471No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA60.5882352941176471No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
ACGCAGAGTGAATGGGCATGAGCACAAAAAAAA40.39215686274509803No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC40.39215686274509803No Hit
GCATGAGCACTGTACGTACTCTGCGTTGATACC40.39215686274509803No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA40.39215686274509803No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GAATGGGCACTGTACGTAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GTGAATGGGCACTGTACAAAAAAAAAAAAAAAA30.29411764705882354No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA30.29411764705882354No Hit
GAATGGGCACTGTACGTACTCAAAAAAAAAAAA30.29411764705882354No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC30.29411764705882354No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC30.29411764705882354No Hit
CCCCACGCCATGAGCACTGTACGTACTCTGCGT20.19607843137254902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20.19607843137254902No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA20.19607843137254902No Hit
GCTAACATGAGCACTGTACGTACTCTGCGTTGA20.19607843137254902No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGCATGAGCAAAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGCATGAGCACTGAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGCACTGTACAAAAAAAAA20.19607843137254902No Hit
GAATGGGCACTGTACGAACAAAAAAAAAAAAAA20.19607843137254902No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC20.19607843137254902No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTACGACAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGCACTGTACGTACCTGAAAAAAAAAAA20.19607843137254902No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GCACTGTACGTACCTAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA20.19607843137254902No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA20.19607843137254902No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GGGTACGTACTCTGCGTACATACCAAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGCATGAGCAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGCATGAGCACTGTACGTA20.19607843137254902No Hit
CCCAACATGAGCACTGTACGTACTCTGCGTTGA20.19607843137254902No Hit
GAGTGAATGGGCACTGTACGTACCAAAAAAAAA20.19607843137254902No Hit
GAATGGGCACTGTACGAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CATGAGCACTGTACGTACTCTGCGTGGATACCA20.19607843137254902No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA20.19607843137254902No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT20.19607843137254902No Hit
GTGAATGGGGCATGAGCAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CCCTCATGAGCACTGTACGTACTCTGCGTTGAT20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)