FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_79.3520000005d2fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_79.3520000005d2fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35469
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA1310.3693366037948631No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA1190.33550424314189853No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA1150.3242267895909104No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA1010.2847557021624517No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA950.26783952183596943No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA900.25374270489723416No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA860.24246525134624602No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA820.23118779779525783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.22554907101976374No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA660.1860779835913051No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA630.17761989342806395No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA630.17761989342806395No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA620.1748005300403169No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA620.1748005300403169No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA610.17198116665256985No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA560.15788434971383464No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA540.1522456229383405No Hit
GGACATCGCAAAAAAAAAAAAAAAAAAAAAAAA530.1494262595505935No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA510.14378753277509937No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA480.13532944261185822No Hit
GTATCAACGCAGAGTGAATGGGATGTCTGACGT470.1325100792241112No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA460.12969071583636416No Hit
GAGTACGTCAAAAAAAAAAAAAAAAAAAAAAAA450.12687135244861708No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT450.12687135244861708No Hit
CATCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA450.12687135244861708No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA440.12405198906087006No Hit
ACGCAGAGTGAATGGGACATCGCAAAAAAAAAA380.10713580873438777No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA370.10431644534664072No Hit
AACGCAGAGTGAATGGGATGTCTGACGTACTCA370.10431644534664072No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA370.10431644534664072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCT356.3494044E-627.026
CAACTCA306.967935E-527.01
GTATCAT200.008500257527.01
AACTCAG306.967935E-527.02
ACTTACT356.3494044E-627.024
CTTACTC356.3494044E-627.025
TCAGAGT306.967935E-527.05
CTCAGAG306.967935E-527.04
TATCATC200.008500257527.02
TCGCAGA200.008500257527.07
ATCATCG200.008500257527.03
ATCGCAG200.008500257527.06
GACTTAC356.3494044E-627.023
CTGACTT451.6216563E-624.021
TGACTTA401.7888382E-523.62522
ACTCAGA401.7888382E-523.6253
CGCTGAG300.002221926822.52
ACGCTGA300.002221926822.51
GCTGAGT300.002221926822.53
TCTGACT504.0506384E-621.59999820
Produced by FastQC (version 0.11.2)