FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_78.3520000005d2d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_78.3520000005d2d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26225
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA2050.7816968541468066No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA1060.4041944709246902No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1030.3927550047664442No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT840.3203050524308866No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA830.31649189704480457No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA800.3050524308865586No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA730.2783603431839848No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA720.27454718779790277No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA630.2402287893231649No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA580.22116301239275502No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA570.21734985700667303No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA570.21734985700667303No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC570.21734985700667303No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA560.21353670162059102No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.2059103908484271No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA540.2059103908484271No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA530.2020972354623451No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA520.1982840800762631No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA500.19065776930409914No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA480.18303145853193517No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT410.1563393708293613No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCACACT350.1334604385128694No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA340.12964728312678742No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA340.12964728312678742No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA330.12583412774070543No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA330.12583412774070543No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC320.12202097235462345No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCAAAAA310.11820781696854146No Hit
TCGTCACACTACGTACTCAAAAAAAAAAAAAAA300.11439466158245949No Hit
GAGTGAATGGGGTTCGTCAAAAAAAAAAAAAAA300.11439466158245949No Hit
TATCAACGCAGAGTGAATGGGTTCGTCAAAAAA290.11058150619637751No Hit
GTGAATGGGGTTCGTCACACAAAAAAAAAAAAA280.10676835081029551No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA270.10295519542421355No Hit
GTATCAACGCAGAGTGAATGGGTTCGTCACACT270.10295519542421355No Hit
GTGAATGGGGTTCGTCACAAAAAAAAAAAAAAA270.10295519542421355No Hit
GTATCAACGCAGAGTGAATGGGGTTCGTCACAC270.10295519542421355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA608.383158E-720.2527
TGATACC651.8060255E-618.69230726
TTGATAC651.8060255E-618.69230725
GTTGATA703.6666534E-617.35714124
TCTGCGT5550.016.5405427
CGTTGAT757.0730075E-616.19999923
TATCAAC1602.1645974E-1013.51
ATCAACG1801.0004442E-1012.752
TCAACGC1851.5825208E-1012.4054053
GCGTTGA1101.870949E-512.27272722
CTCTGCG7900.011.62025326
TGGGTTC1401.4226862E-611.5714276
ATGGGTT1308.049801E-611.4230775
CCGTTCG2451.0913936E-1111.0204097
ACTCTGC8650.010.61271625
CAACGCA2202.8212526E-910.4318184
CGTTCGT5050.010.4257438
TGCGTTG1701.269178E-610.32352827
GCGTTCG1050.002210282510.2857147
AATGGGT1452.7010497E-510.2413794
Produced by FastQC (version 0.11.2)