FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_77.3520000005d2b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_77.3520000005d2b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40686
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA4161.022464729882515No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC3350.8233790493044291No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA2710.6660767831686575No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA2310.5677628668338004No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA2180.5358108440249717No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA1620.3981713611561717No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC1460.35884579462222876No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA1400.3440987071720002No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.331809467630143No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA920.22612200757017156No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA880.21629061593668583No Hit
GTGAATGGGGTCCAAAAAAAAAAAAAAAAAAAA790.19416998476134295No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA780.19171213685297153No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA770.1892542889446001No Hit
ACGCAGAGTGAATGGGAGGTCCAAAAAAAAAAA770.1892542889446001No Hit
TATCAACGCAGAGTGAATGGGAGGTCCAAAAAA700.1720493535860001No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.16713365776925723No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA670.1646758098608858No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA640.15730226613577153No Hit
TATCAACGCAGAGTGAATGGGGTCCAAAAAAAA640.15730226613577153No Hit
GAGTGAATGGGGTCCAAAAAAAAAAAAAAAAAA640.15730226613577153No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA630.15484441822740008No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.15484441822740008No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA620.15238657031902864No Hit
GTGAATGGGGTACGTAAGGTCCAAAAAAAAAAA590.14501302659391438No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT590.14501302659391438No Hit
TATCAACGCAGAGTGAATGGGGGTCCAAAAAAA580.14255517868554293No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.13763948286880007No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT530.13026593914368578No Hit
GTGAATGGGTGCTTGGACCAAAAAAAAAAAAAA520.12780809123531436No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA510.12535024332694295No Hit
ACGCAGAGTGAATGGGGTCCAAAAAAAAAAAAA510.12535024332694295No Hit
GTGAATGGGAGTACGTAAGGTCCAAAAAAAAAA510.12535024332694295No Hit
TACGTACTCTGCGTTGATACCAAAAAAAAAAAA510.12535024332694295No Hit
GTGAATGGGAAGGTCCAAAAAAAAAAAAAAAAA490.12043454751020007No Hit
GTGAATGGGTGCTTGGAAAAAAAAAAAAAAAAA480.11797669960182863No Hit
AACGCAGAGTGAATGGGAGGTCCAAAAAAAAAA480.11797669960182863No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.11551885169345721No Hit
CGTAAGGTCCAAAAAAAAAAAAAAAAAAAAAAA450.11060315587671433No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA440.10814530796834292No Hit
GAGTGAATGGGGGTCCAAAAAAAAAAAAAAAAA430.10568746005997148No Hit
GCTTGGACCTTACGTACTCTGCGTTTATACCAC410.10077176424322865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG356.379689E-627.02
TCTACGC356.379689E-627.03
TACGCAG405.841175E-727.05
TATCTAC356.379689E-627.01
GTATCAT551.3622412E-824.5454561
CAACTCA401.7972889E-523.6251
TCAGAGT401.7972889E-523.6255
CTACGCA401.7972889E-523.6254
TATCATC653.1595846E-922.8461532
TCGCAGA603.4309778E-822.57
ATCATCG603.4309778E-822.53
ATCGCAG603.4309778E-822.56
TCATCGC603.4309778E-822.54
CATCGCA603.4309778E-822.55
AACTCAG454.4640223E-521.02
CTCAGAG454.4640223E-521.04
ACTCAGA501.0038754E-418.9000023
TATCAAC2750.016.6909081
AGTACGT1002.456909E-816.22
ATACCAC952.453744E-715.63157927
Produced by FastQC (version 0.11.2)