FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_76.3520000005d2ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_76.3520000005d2ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26105
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA2450.9385175253782799No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT1320.505650258571155No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA940.3600842750430952No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA870.33326948860371575No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA730.2796399157249569No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT720.27580923194790274No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA720.27580923194790274No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA640.24516376173146906No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA570.21834897529208966No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA530.20302624018387283No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA520.19919555640681863No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA490.187703505075656No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA470.1800421375215476No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.1723807699674392No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA440.16855008619038497No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA420.16088871863627657No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA400.15322735108216817No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCT380.14556598352805974No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA380.14556598352805974No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC340.13024324841984294No Hit
GAGTGAATGGGATCTCGTCCTCAAAAAAAAAAA330.12641256464278874No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCA320.12258188086573453No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCTC320.12258188086573453No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA320.12258188086573453No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCAAAA320.12258188086573453No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA300.11492051331162612No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA290.11108982953457192No Hit
AACGCAGAGTGAATGGGATCTCGTCCAAAAAAA290.11108982953457192No Hit
ATCTCGTCCTCAAAAAAAAAAAAAAAAAAAAAA280.10725914575751772No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA280.10725914575751772No Hit
GTCCTCTACGTACTCAAAAAAAAAAAAAAAAAA280.10725914575751772No Hit
GATCTCGTCCTCAAAAAAAAAAAAAAAAAAAAA270.1034284619804635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG300.002206182322.52
CTGAGTG300.002206182322.54
ACGCTGA300.002206182322.51
GCTGAGT300.002206182322.53
TGAGTGA300.002206182322.55
CGAATCT350.00539196819.2857154
TATCAAC2550.016.9411751
GCGTTGA1800.015.7527
ATCAACG2750.015.2181832
TCAACGC2750.014.7272743
GATACCA657.302707E-414.53846127
CAACGCA2800.014.4642864
TGCGTTG3600.014.2527
TGATACC700.001279236313.526
TTGATAC700.001279236313.525
AACGCAG3050.013.2786885
GTTGATA750.002151055512.59999924
CGTTGAT800.003490449911.812523
CGATCTC850.0054893211.1176467
CTGCGTT4800.010.9687526
Produced by FastQC (version 0.11.2)