FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_75.3520000005d283.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_75.3520000005d283.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31086
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.20909734285530462No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA640.20588046065753074No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT600.19301293186643506No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA520.1672778742842437No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA480.15441034549314803No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA430.13832593450427844No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA430.13832593450427844No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA410.13189217010873064No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA340.10937399472431321No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA330.10615711252653928No Hit
GGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.10615711252653928No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA320.10294023032876537No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA320.10294023032876537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAG356.2688305E-627.0258082
ACTCAGA356.2688305E-627.0258083
GTATCAT257.613735E-427.0258051
ATCATCG257.613735E-427.0258053
ATCGCAG257.686055E-426.9822866
CTCAGAG401.7885774E-523.60954
TCAGAGT351.9864179E-423.1276745
TATCATC300.002203385622.5215052
TCGCAGA300.00222420222.4852397
TCATCGC300.00222420222.4852394
CATCGCA300.00222420222.4852395
CAACTCA454.3875698E-521.0200731
TGCGTTG2450.019.33533327
GCGTTGA707.133512E-515.468266527
CTGCGTT3350.014.52132826
TATCAAC1851.6189006E-1012.4172621
TCTGCGT6250.012.343677527
ATCAACG2151.9736035E-910.6846212
TCAACGC2306.000846E-99.9877993
CTCTGCG7900.09.74981626
Produced by FastQC (version 0.11.2)