FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_74.3520000005d25d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_74.3520000005d25d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43631
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5211.1941051087529508No Hit
GACACAGACAGTACGTACTCTGCGTTGATACCA5171.1849373152116613No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA4230.9694941669913594No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA4050.9282390960555569No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA4030.9236551992849121No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT3840.8801081799637872No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA3090.70821205106461No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2930.6715408768994523No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA2790.6394535995049392No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA2530.5798629414865577No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA2490.5706951479452682No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA2410.5523595608626893No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA2350.5386078705507552No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA1920.4400540899818936No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA1890.4331782448259265No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA1840.42171850289931473No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA1610.36900369003690037No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGT1580.3621278448809333No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA1520.3483761545689991No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.3483761545689991No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA1460.33462446425706494No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA1460.33462446425706494No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA1420.32545667071577544No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA1280.29336939332126244No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA1270.29107744493594007No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGTA1200.2750338062386835No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA1190.27274185785336114No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA1190.27274185785336114No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.27044990946803876No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGT1090.24982237400013751No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA1070.24523847722949277No Hit
GACAGTACAAAAAAAAAAAAAAAAAAAAAAAAA1060.24294652884417042No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA1050.24065458045884808No Hit
TATCTACGCAGAGTGAATGGGACACAGACAAAA1050.24065458045884808No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA1040.2383626320735257No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA1030.23607068368820336No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA1020.23377873530288099No Hit
CAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.2314867869175586No Hit
CAACTCAGAGTGAATGGGACACAGACAGAAAAA920.21085925144965736No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA900.20627535467901262No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA890.20398340629369024No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA870.19939950952304555No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA820.18793976759643374No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.183355870825789No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.1764800256698219No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA760.17418807728449956No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.17418807728449956No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA750.1718961288991772No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA750.1718961288991772No Hit
GTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.1650202837432101No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGA720.1650202837432101No Hit
ACGCAGAGTGAATGGGACACAGACAGTACAAAA720.1650202837432101No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAG710.16272833535788772No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.15585249020192066No Hit
AGACAGTACGTACTCAAAAAAAAAAAAAAAAAA660.15126859343127594No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA660.15126859343127594No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.15126859343127594No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC630.14439274827530885No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA630.14439274827530885No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.14210079988998647No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA620.14210079988998647No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA590.13522495473401938No Hit
CAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.13293300634869704No Hit
ACGCAGAGTGAATGGGACACAGACAGTACGTAC580.13293300634869704No Hit
GAATGGGACACAGACAGTAAAAAAAAAAAAAAA580.13293300634869704No Hit
GAATGGGGACACAGACAGAAAAAAAAAAAAAAA570.13064105796337466No Hit
GAGTGAATGGGACACAGACAGTAAAAAAAAAAA560.1283491095780523No Hit
ATGGGACACAGACAAAAAAAAAAAAAAAAAAAA560.1283491095780523No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGAA540.12376521280740757No Hit
GGACACAGACAGAAAAAAAAAAAAAAAAAAAAA530.12147326442208521No Hit
TATCAACGCAGAGTGAATGGGGACACAGACAGT520.11918131603676285No Hit
CAACTCAGAGTGAATGGGACACAGACAGTACAA510.11688936765144049No Hit
AACGTAGAGTGAATGGGACACAGACAAAAAAAA500.11459741926611812No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA500.11459741926611812No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA480.1100135224954734No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA470.10772157411015103No Hit
AATGGGACACAGACAAAAAAAAAAAAAAAAAAA470.10772157411015103No Hit
GGACACAGACAAAAAAAAAAAAAAAAAAAAAAA460.10542962572482868No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA460.10542962572482868No Hit
CAACTCAGAGTGAATGGGACACAGACAGTAAAA460.10542962572482868No Hit
GAATGGGACACAGACAGTACAAAAAAAAAAAAA450.10313767733950631No Hit
GGACACAGACAGTACAAAAAAAAAAAAAAAAAA450.10313767733950631No Hit
ACGCAGAGTGAATGGGACACAGAAAAAAAAAAA450.10313767733950631No Hit
AACGCAGAGTGAATGGGACACAGACAGTAAAAA440.10084572895418395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA1150.027.01
GTATCAT504.958565E-927.01
TATTAAC200.00852131727.02
AACTCAG1200.025.8749982
ATCTACG1050.025.7142852
TATCTAC1050.025.7142851
CTACGCA1050.025.7142854
TCTACGC1000.025.6500023
ACTCAGA1250.024.843
CTCAGAG1250.024.844
TCAGAGT1150.024.6521745
TATCATC551.3680619E-824.5454542
TACGCAG1050.024.4285725
CACTGCT501.4932266E-724.30000127
CCACTGC501.4932266E-724.30000126
TACCACT553.7465907E-722.0909124
ACCACTG608.651823E-720.24999825
GGTATCA559.323376E-619.6363641
ATACCAC703.7823265E-617.35714323
TCGCAGA901.3327553E-716.5000027
Produced by FastQC (version 0.11.2)