FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_71.3520000005d20c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_71.3520000005d20c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39490
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA7271.8409723980754622No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA4731.1977715877437325No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG3620.916687769055457No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA3580.9065586224360598No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA2390.6052165105089896No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA1820.46087617118257784No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC1790.4532793112180299No Hit
GGATAGCAGATACGTACTCTGCGTTGATACCAC1650.4178272980501393No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.38997214484679665No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA1360.3443909850595087No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA1330.33679412509496076No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC1310.3317295517852621No Hit
GTATCATCGCAGAGTGAATGGGATAGCAGATAC1260.31906811851101546No Hit
TATCTACGCAGAGTGAATGGGATAGCAGATACG1230.31147125854646746No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT1190.30134211192707017No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA1150.2912129653076728No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA1010.2557609521397822No Hit
CAACTCAGAGTGAATGGGATAGCAGATACGTAC970.2456318055203849No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA940.23803494555583693No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA820.20764750569764495No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT800.2025829323879463No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA800.2025829323879463No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA800.2025829323879463No Hit
TATCAACGCAGAGTGAATGGGTAGCAGATACGT800.2025829323879463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.200050645733097No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA720.1823246391491517No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT650.16459863256520638No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA620.1570017726006584No Hit
GAATGGGATAGCAGATACGTACTCAAAAAAAAA590.1494049126361104No Hit
GGATAGCAGAAAAAAAAAAAAAAAAAAAAAAAA560.1418080526715624No Hit
GGTATCAACGCAGAGTGAATGGGATAGCAGATA550.1392757660167131No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA540.13674347936186376No Hit
CAACGCAGAGTGAATGGGATAGCAGATACGTAC540.13674347936186376No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.13421119270701443No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC510.12914661939731578No Hit
GTGAATGGGTAGCAGATACGTACTCAAAAAAAA500.12661433274246645No Hit
GAATGGGATAGCAGATACGTACAAAAAAAAAAA470.11901747277791846No Hit
ACGCAGAGTGAATGGGTAGCAGATACGTACTCA470.11901747277791846No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA460.11648518612306913No Hit
CAGATACGTACTCAAAAAAAAAAAAAAAAAAAA460.11648518612306913No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.1139528994682198No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTA430.10888832615852115No Hit
GTGAATGGGATAGCAGATACGTAAAAAAAAAAA420.10635603950367183No Hit
GGATAGCAGATACGTACTAAAAAAAAAAAAAAA420.10635603950367183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG257.672383E-427.0127093
TATTAAC257.672383E-427.0127092
TTAACGC257.672383E-427.0127094
GTATCAT604.1836756E-1127.0127071
TATCATC554.5110937E-1027.0127072
AATCGGA200.00854485726.9784286
TATCTAC551.3526005E-824.5570051
GTATTAA300.002220185722.5105881
CACCCGG300.002236681322.4820235
CAACTCA553.7106292E-722.1013051
CTACGCA657.9475285E-820.7790054
GTATCAA1003.6379788E-1220.2595311
ATCTACG608.569241E-720.259532
TCTACGC608.569241E-720.259533
TAACGCA404.93658E-420.233825
ATCATCG751.6323611E-819.8093173
ATCGCAG751.6565536E-819.784186
CATCGCA751.6565536E-819.784185
TACGCAG701.760418E-719.2703065
TCAGAGT501.0090443E-418.88495
Produced by FastQC (version 0.11.2)