FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_70.3520000005d1d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_70.3520000005d1d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44467
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC6101.371803809566645No Hit
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA3600.8095891335147413No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA3360.7556165246137585No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA2630.5914498392066027No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA2350.5284817954887894No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA2250.5059932084467132No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA1860.4182877189826163No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA1720.3868036971237097No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC1570.3530708165605955No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA1390.3125913598848584No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA1380.3103425011806508No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC1370.3080936424764432No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.2946004902511975No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA1300.2923516315469899No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA1260.28335619673015944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.25412103357546045No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA1120.25187217487125285No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC1110.24962331616704522No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA1080.24287674005442236No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA1040.2338813052375919No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC990.22263701171655387No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA980.22038815301234624No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA910.20464614208289292No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC870.19565070726606246No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA870.19565070726606246No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA820.1844064137450244No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACGTAC820.1844064137450244No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA810.1821575550408168No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACT800.17990869633660916No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC790.17765983763240153No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA750.1686644028155711No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA730.16416668540715587No Hit
GTGAATGGGGTAGTTCTGACACGTACTCAAAAA720.16191782670294824No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG720.16191782670294824No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA710.15966896799874064No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCAAAAA690.1551712505903254No Hit
GAATGGGTAGTTCTGACACAAAAAAAAAAAAAA690.1551712505903254No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA670.15067353318191018No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA660.14842467447770258No Hit
ACGCAGAGTGAATGGGGTAGTTCAAAAAAAAAA650.14617581577349495No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACAAAA640.14392695706928735No Hit
GTATCATCGCAGAGTGAATGGGGTAGTTCTGAC640.14392695706928735No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA630.14167809836507972No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA620.1394292396608721No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA620.1394292396608721No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA570.12818494613983403No Hit
GTTCTGACACGAAAAAAAAAAAAAAAAAAAAAA570.12818494613983403No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.12143837002721118No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA530.11918951132300357No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA520.11694065261879595No Hit
GTGAATGGGTAGTTCTGAAAAAAAAAAAAAAAA510.11469179391458834No Hit
ATCAACGCAGAGTGAATGGGGTAGTTCTGACAC500.11244293521038073No Hit
GTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1079452178019655No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC480.1079452178019655No Hit
CAACTCAGAGTGAATGGGTAGTTCTGACACAAA470.10569635909775789No Hit
GACACGTACAAAAAAAAAAAAAAAAAAAAAAAA460.10344750039355027No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAA460.10344750039355027No Hit
GGGTAGTTCTGACACGTACAAAAAAAAAAAAAA450.10119864168934266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT455.3876647E-827.0000021
TATCATC455.3876647E-827.0000022
CCCGAGG200.00852318427.04
CCGAGGT200.00852318427.05
ATCTACG751.8189894E-1225.22
TCTACGC751.8189894E-1225.23
TACGCAG751.8189894E-1225.25
TATCTAC751.8189894E-1225.21
CTACGCA751.8189894E-1225.24
CAACTCA501.4946818E-724.3000011
TCGCAGA653.1795935E-922.8461557
ATCGCAG653.1795935E-922.8461556
ATCATCG603.4493496E-822.53
TCATCGC603.4493496E-822.54
CATCGCA603.4493496E-822.55
AACTCAG608.660154E-720.252
TATCAAC4100.018.1097561
TGCGTTG5000.017.8227
ACTCAGA703.7859118E-617.3571433
TCAGAGT703.7859118E-617.3571435
Produced by FastQC (version 0.11.2)