FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_7.3520000005ca07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_7.3520000005ca07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10981
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA710.6465713505145251No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA680.6192514342956015No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA600.5463983243784719No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA520.4735452144613423No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA470.4280120207631364No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA460.4189053820234951No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA410.3733721883252892No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.3460522721063656No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC330.30051907840815956No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA320.2914124396685184No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA320.2914124396685184No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA280.25498588470995354No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA260.23677260723067114No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA230.20945269101174754No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA190.1730261360531828No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA190.1730261360531828No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA190.1730261360531828No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA180.16391949731354155No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA170.15481285857390037No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA170.15481285857390037No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA160.1457062198342592No Hit
GTGAATGGGCATTGCTGAAGACGTAAAAAAAAA150.13659958109461798No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG150.13659958109461798No Hit
GTGAATGGGGCATTGCTGAAGACAAAAAAAAAA150.13659958109461798No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA150.13659958109461798No Hit
GCATTGCTGAAAAAAAAAAAAAAAAAAAAAAAA150.13659958109461798No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA140.12749294235497677No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACAAA130.11838630361533557No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC130.11838630361533557No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA130.11838630361533557No Hit
GCATTGCTGAAGACGTACAAAAAAAAAAAAAAA130.11838630361533557No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA130.11838630361533557No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA130.11838630361533557No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA120.10927966487569438No Hit
GCATTGCTGAAGACGAAAAAAAAAAAAAAAAAA120.10927966487569438No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10927966487569438No Hit
AACGTAGAGTGAATGGGCATTGCTGAAAAAAAA120.10927966487569438No Hit
GTCTTCAGCAAAAAAAAAAAAAAAAAAAAAAAA110.1001730261360532No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAA110.1001730261360532No Hit
CCCCCGCATTGCTGAAGACGTACTCTGCGTTGA110.1001730261360532No Hit
GTGAATGGGGCATTGCTGAAGACGTACTCAAAA110.1001730261360532No Hit
GCTGAAGACGTACTAAAAAAAAAAAAAAAAAAA110.1001730261360532No Hit
GTGAATGGGCATTGCTGAAGACGAAAAAAAAAA110.1001730261360532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC509.2095215E-518.9000021
GCGTTGA703.2849966E-617.35714327
ATCAACG551.9126704E-417.1818182
TCTGCGT2300.015.84782727
TGCGTTG801.1711063E-515.187526
CTGCGTT1009.594062E-512.15000125
CTCTGCG3000.012.1526
ACTCTGC3350.010.88059725
ACGCAGA1751.4985562E-610.0285711
TACTCTG3550.09.88732424
GTACTCT3700.09.48648623
GCAGAGT1606.87883E-59.281253
CGCAGAG1751.7218685E-59.2571422
CAGAGTG1659.6354284E-59.04
CGCATTG1802.404414E-59.08
ACGTACT4500.08.721
CGTACTC4300.08.47674422
GCATTGC6350.08.2913389
AGAGTGA1802.4848848E-48.255
CATTGCT6300.08.14285710
Produced by FastQC (version 0.11.2)