FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_68.3520000005d191.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_68.3520000005d191.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30486
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGGAGACTACGTACTCTGCGTTGATACCA3241.0627829167486715No Hit
GTAGGAGACTACGTACTCTGCGTTGATACCACT3081.010299809748737No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA2510.8233287410614708No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA2200.721642721249098No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA1470.4821885455618973No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA1330.4362658269369547No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA1160.38050252574952437No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA1130.37066194318703666No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA1100.360821360624549No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA970.3181788361871023No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA940.3083382536246146No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA890.2919372826871351No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA850.27881650593715146No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.2460145640621925No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA690.22633339893721707No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA680.22305320474972118No Hit
GTATCATCGCAGAGTGAATGGGACGTAGGAGAC590.1935314570622581No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC580.19025126287476218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.1869710686872663No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA560.1836908744997704No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA550.1804106803122745No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC530.17385029193728269No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA530.17385029193728269No Hit
AACGCAGAGTGAATGGGACGTAGGAGAAAAAAA520.1705700977497868No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.16400970937479498No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA490.16072951518729908No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA450.14760873843731548No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA450.14760873843731548No Hit
GACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAA440.14432854424981958No Hit
GAATGGGACGTAGGAGAAAAAAAAAAAAAAAAA440.14432854424981958No Hit
GAACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA420.1377681558748278No Hit
CAACTCAGAGTGAATGGGACGTAGGAGAAAAAA410.1344879616873319No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA410.1344879616873319No Hit
TATCTACGCAGAGTGAATGGGACGTAGGAGACA410.1344879616873319No Hit
GTATCATCGCAGAGTGAATGGGACGTAGGAGAA400.131207767499836No Hit
GTGAATGGGAACGTAGGAGACAAAAAAAAAAAA390.1279275733123401No Hit
CAACTCAGAGTGAATGGGACGTAGGAGACAAAA390.1279275733123401No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA390.1279275733123401No Hit
GTGAATGGGAACGTAGGAGAAAAAAAAAAAAAA390.1279275733123401No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAA380.1246473791248442No Hit
ACGCAGAGTGAATGGGACGTAGGAAAAAAAAAA370.1213671849373483No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.1082464081873647No Hit
GTGAATGGGGACGTAGGAAAAAAAAAAAAAAAA330.1082464081873647No Hit
CCACGTAGGAGACTACGTACTCTGCGTTGATAC310.10168601981237288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT653.6379788E-1227.0427631
GAGTAAA200.00841625927.042761
GTAGAGT257.655919E-426.9983584
AGTAAAT200.008484215526.9983582
TAGAGTG257.655919E-426.9983585
TACCACT200.008484215526.99835827
TATCATC701.0913936E-1125.0699042
TCGCAGA855.456968E-1222.2339447
ATCGCAG855.456968E-1222.2339446
CATCGCA855.456968E-1222.2339445
ATCATCG806.184564E-1121.9361653
TCATCGC806.184564E-1121.9361654
TATCTAC503.9642946E-621.634211
ATCTACG504.0215655E-621.5986862
TCTACGC504.0215655E-621.5986863
CTACGCA454.418737E-520.9987224
CGTAGAG404.8767123E-420.2487683
CAACTCA350.00536314619.316261
TACGCAG509.937927E-518.8988515
TATCAAC2250.018.028511
Produced by FastQC (version 0.11.2)