FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_62.3520000005d0e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_62.3520000005d0e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44409
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA8721.9635659438402127No Hit
CAACAGAGCATACGTACTCTGCGTTGATACCAC3310.7453444121687045No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA2630.5922222972820824No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA2360.5314238104888649No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.403071449480961No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.3760498997950866No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA1560.3512801459163683No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA1530.3445247584948997No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA1500.3377693710734311No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA1250.2814744758945259No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.26120831363012004No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC1090.24544574298002658No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA1030.23193496813708933No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA990.22292778490846452No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA960.2161723974869959No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA950.21392060167983967No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC940.2116688058726835No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAA920.20716521425837106No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT900.20266162264405865No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT890.20040982683690242No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA870.19590623522259001No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.1914026436082776No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA800.18014366457249656No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA790.17789186876534036No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAT770.17338827715102795No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA760.17113648134387174No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA730.1643810939224031No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA700.1576257065009345No Hit
TATCTACGCAGAGTGAATGGGCAACAGAGCATA680.15312211488662208No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA670.15087031907946588No Hit
GTGAATGGGCAACAGAGCATAAAAAAAAAAAAA660.14861852327230968No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA660.14861852327230968No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAA650.14636672746515347No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA610.13735954423652863No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.13510774842937243No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA590.13285595262221622No Hit
GGTATCAACGCAGAGTGAATGGGCAACAGAGCA590.13285595262221622No Hit
GGGCAACAGAGCATACGTACTCAAAAAAAAAAA580.13060415681506002No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.11709338197212277No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA520.11709338197212277No Hit
GGGCAACAGAGCATAAAAAAAAAAAAAAAAAAA500.11258979035781035No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA490.11033799455065414No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA490.11033799455065414No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA480.10808619874349795No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC480.10808619874349795No Hit
GGGCAACAGAGCATACGTACTCTGCGTTGATAC480.10808619874349795No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA480.10808619874349795No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA470.10583440293634175No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA470.10583440293634175No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAT460.10358260712918553No Hit
CAACAGAGCATACGTACTCTGCGTTTATACCAC460.10358260712918553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT257.6864E-427.0135424
ACGTAGA257.6864E-427.0135422
CGTAGAG257.6864E-427.0135423
TATCATC750.027.0135422
TCGCAGA750.027.0135427
ATCATCG750.027.0135423
ATCGCAG750.027.0135426
TCATCGC750.027.0135424
CATCGCA750.027.0135425
CAACTCA200.008549063526.9830911
CAACACA200.008549063526.9830911
AACGTAG257.737367E-426.983091
TCTACGC653.1614036E-922.8576133
CTACGCA653.1614036E-922.8576134
TATCTAC653.2032403E-922.8318441
GTATCAT950.022.72261
TAGAGTG300.002224185522.5112865
ATCTACG707.4378477E-921.2249262
TACGCAG707.4378477E-921.2249265
ATGCAAC350.005435271719.2953877
Produced by FastQC (version 0.11.2)