FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_58.3520000005d05f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_58.3520000005d05f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37506
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA3510.9358502639577668No Hit
ATGCAGAGACGTACGTACTCTGCGTTGATACCA3130.8345331413640484No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA2340.6239001759718446No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA2160.5759078547432411No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA1910.5092518530368475No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA1660.44259585133045376No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT1420.3786060896923159No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA1330.3546099290780142No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA1250.3332800085319682No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1220.325281288327201No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT1090.2906201674398763No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.2799552071668533No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA1040.27728896709859757No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT840.22396416573348266No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA810.21596544552871538No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA810.21596544552871538No Hit
GACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.1973017650509252No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.19463552498266945No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA690.18397056470964646No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA660.17597184450487924No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA650.1733056044366235No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.157308164027089No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA570.1519756838905775No Hit
GTATCATCGCAGAGTGAATGGGATGCAGAGACG550.14664320375406603No Hit
ATGCAGAGACGTACGAAAAAAAAAAAAAAAAAA550.14664320375406603No Hit
GGAGACGTACGTACTCAAAAAAAAAAAAAAAAA520.13864448354929879No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.13597824348104304No Hit
ATGCAGAGACGTAAAAAAAAAAAAAAAAAAAAA510.13597824348104304No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA490.13064576334453154No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1279795232762758No Hit
GTATCAACGCAGAGTGAATGGGATGCAGAGACG470.12531328320802004No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA470.12531328320802004No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACAA460.1226470431397643No Hit
TATCAACGCAGAGTGAATGGGAGAGACGTACGT450.11998080307150856No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGA450.11998080307150856No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA450.11998080307150856No Hit
GTGAATGGGATGCAGAGACGAAAAAAAAAAAAA440.11731456300325281No Hit
AGACGTACGTACTCAAAAAAAAAAAAAAAAAAA420.11198208286674133No Hit
GTGAATGGGAATGCAGAGACAAAAAAAAAAAAA400.10664960273022983No Hit
CCCCCATGCAGAGACGTACGTACTCTGCGTTGA400.10664960273022983No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA390.10398336266197408No Hit
GGGGAGACGTACGTACTCAAAAAAAAAAAAAAA390.10398336266197408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT504.9094524E-927.01
CAACTCA604.1836756E-1126.9999981
AACTCAG651.1459633E-1024.9230772
TATCATC551.3549652E-824.5454542
ATCTACG401.7923629E-523.6252
TCTACGC401.7923629E-523.6253
TACGCAG401.7923629E-523.6255
TATCTAC401.7923629E-523.6251
CTCAGAG702.8921932E-1023.1428594
ACTCAGA756.85759E-1021.63
TCAGAGT707.386916E-921.2142875
CTACGCA454.451903E-521.0000024
GGTATCA454.451903E-521.0000021
GTATCAA1250.018.361
GCGTTGA2650.018.33962227
TCGCAGA753.5943594E-718.0000027
ATCATCG753.5943594E-718.0000023
ATCGCAG753.5943594E-718.0000026
TCATCGC753.5943594E-718.0000024
CATCGCA753.5943594E-718.0000025
Produced by FastQC (version 0.11.2)