FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_57.3520000005d035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_57.3520000005d035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33127
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC3200.9659794125637697No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC1690.5101578772602409No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1530.46185890663205237No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1330.4014851933468168No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA1240.37431702236846076No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1240.37431702236846076No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA970.2928125094333927No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT750.22640142481963355No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA710.2143266821625864No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC570.1720650828629215No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA560.16904639719865971No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA500.15093428321308902No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC500.15093428321308902No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA490.14791559754882724No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA490.14791559754882724No Hit
ACGCAGAGTGAATGGGCTACGTACCGAAAAAAA450.1358408548917801No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.13282216922751836No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA410.123766112234733No Hit
ACTACGTACCGTAAAAAAAAAAAAAAAAAAAAA410.123766112234733No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA390.11772874090620943No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA390.11772874090620943No Hit
GTGAATGGGCTACGTACAAAAAAAAAAAAAAAA370.11169136957768588No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA350.10565399824916233No Hit
CTCTACGTACCGTAACGTACTCTGCGTTGATAC350.10565399824916233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTA257.6671987E-427.05
TGATACC855.456968E-1222.23529227
TTGATAC1154.4201443E-1016.43478226
ATCCTAC500.00218611716.26
TATCAAC1700.015.8823521
ATCAACG1800.015.02
TCTGCGT7900.013.67088627
CTCCTAC802.2791285E-413.57
TCAACGC1901.8189894E-1213.53
ACCTACG750.002172530212.5999997
CAACGCA2057.094059E-1111.8536594
GTCTACG800.003524947411.81257
GTTGATA1751.599401E-710.825
CTCTGCG10350.010.43478326
CGTTGAT2009.02437E-810.12524
GTATCAA1258.545509E-49.721
ACTCTGC11350.09.39647725
AACGCAG2451.7382263E-89.3673475
TACTCTG12050.08.96265624
ACGTACT12400.08.92741921
Produced by FastQC (version 0.11.2)