HTC23BCXX_l02n02_kb_plate3_55.3520000005cff3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HTC23BCXX_l02n02_kb_plate3_55.3520000005cff3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37207
Sequences flagged as poor quality	0
Sequence length	33
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA	253	0.6799795737361249	No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA	232	0.6235385814497272	No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA	186	0.49990593167952263	No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA	150	0.40314994490284084	No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA	146	0.39239927970543176	No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA	131	0.3520842852151477	No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG	124	0.33327062111968175	No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA	110	0.29564329292874997	No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA	104	0.27951729513263635	No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC	95	0.2553282984384659	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	90	0.24188996694170453	No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA	85	0.22845163544494315	No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA	73	0.19619963985271588	No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA	72	0.1935119735533636	No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA	69	0.1854489746553068	No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA	63	0.16932297685919317	No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA	58	0.1558846453624318	No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA	57	0.15319697906307952	No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA	54	0.1451339801650227	No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA	54	0.1451339801650227	No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA	53	0.14244631386567044	No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT	53	0.14244631386567044	No Hit
GTATCATCGCAGAGTGAATGGGCATTGCTGAAG	50	0.13438331496761363	No Hit
TATCAACGCAGAGTGAATGGGGCATTGCTGAAG	49	0.13169564866826133	No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA	49	0.13169564866826133	No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA	49	0.13169564866826133	No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC	48	0.1290079823689091	No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA	47	0.1263203160695568	No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA	46	0.12363264977020452	No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA	46	0.12363264977020452	No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA	46	0.12363264977020452	No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA	46	0.12363264977020452	No Hit
GCATTGCTGAAGACGTACTCAAAAAAAAAAAAA	45	0.12094498347085227	No Hit
GCTGAAGACGTACTAAAAAAAAAAAAAAAAAAA	45	0.12094498347085227	No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA	45	0.12094498347085227	No Hit
GAGTACGTCTTCAGCAAAAAAAAAAAAAAAAAA	44	0.11825731717149998	No Hit
GTCTTCAGCAAAAAAAAAAAAAAAAAAAAAAAA	43	0.11556965087214771	No Hit
TATCTACGCAGAGTGAATGGGCATTGCTGAAGA	43	0.11556965087214771	No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAA	39	0.10481898567473863	No Hit
GCTGAAGACGTAAAAAAAAAAAAAAAAAAAAAA	39	0.10481898567473863	No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA	39	0.10481898567473863	No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA	38	0.10213131937538635	No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA	38	0.10213131937538635	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTGAG	25	7.676172E-4	27.00539	2
GCTGAGT	25	7.676172E-4	27.00539	3
TGAGTGA	25	7.676172E-4	27.00539	5
ACGCTGA	25	7.7370147E-4	26.969044	1
GCCGCAT	35	1.9851106E-4	23.147476	6
CGCGCAT	35	1.9851106E-4	23.147476	6
AACGTAG	35	2.0033805E-4	23.116322	1
CTGAGTG	30	0.0022212944	22.50449	4
CGTAGAG	40	4.8959744E-4	20.254044	3
CGGCATT	75	1.6298145E-8	19.803953	7
TGGGGTA	35	0.005428371	19.289564	6
GCGTTGA	175	0.0	19.289564	27
CAGGCAT	35	0.005428371	19.289564	6
TCATCGC	35	0.005428371	19.289564	4
ACGTAGA	45	0.0010814952	18.003593	2
AGGCATT	55	2.0737735E-4	17.18525	7
CCGGCAT	50	0.0021900393	16.203234	6
TCTGCGT	1090	0.0	15.360865	27
GCGCATT	110	1.2769779E-6	13.502696	7
TGCGTTG	350	0.0	13.502695	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content