FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_54.3520000005cfd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_54.3520000005cfd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23766
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC2811.1823613565597912No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA1490.6269460573929142No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA970.40814609105444755No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC920.3871076327526719No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA800.33661533282841033No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT790.3324076411680552No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA720.3029537995455693No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC680.28612303290414876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.2777076495834385No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA660.2777076495834385No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA650.2734999579230834No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA630.2650845746023731No Hit
GAGTACGTCTGATCGTAGCAAAAAAAAAAAAAA620.260876882942018No Hit
TATCAACGCAGAGTGAATGGGATCAGACGTACT560.23563073297988724No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA510.2145922746781116No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA440.1851384330556257No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA440.1851384330556257No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA410.1725153580745603No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA360.15147689977278464No Hit
GTATCAACGCAGAGTGAATGGGATCAGACGTAC340.14306151645207438No Hit
GGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA340.14306151645207438No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA340.14306151645207438No Hit
GACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA330.13885382479171926No Hit
GATCGTAGCAAAAAAAAAAAAAAAAAAAAAAAA320.13464613313136414No Hit
GTGAATGGGATCAGACGTACTCAAAAAAAAAAA320.13464613313136414No Hit
ATGCTACGATCAGACGTACTCTGCGTTGATACC320.13464613313136414No Hit
GTGCTACAAAAAAAAAAAAAAAAAAAAAAAAAA320.13464613313136414No Hit
GTCTGATCGTAGCAAAAAAAAAAAAAAAAAAAA310.130438441471009No Hit
CCCCTGCTACGATCAGACGTACTCTGCGTTGAT310.130438441471009No Hit
GCTACGATCAGACGTACTCTGCGTTTATACCAC310.130438441471009No Hit
GAGTGAATGGGATCAGACGTACTCAAAAAAAAA300.12623074981065388No Hit
TGCTACGATCAGACGTACTCTGCGTTGATACCA290.12202305815029874No Hit
ACGCAGAGTGAATGGGATCAGACGTACTCAAAA260.10939998316923336No Hit
GTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.10939998316923336No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA250.10519229150887824No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT240.10098459984852311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAA257.6031394E-426.99999810
CTGCGTA257.6031394E-426.9999989
GCGTAAA300.002200452122.511
AGAATGG350.005378148519.28571315
CGTAAAA350.005378148519.28571312
GTAAAAA500.002161716.19999913
TATCAAC2150.014.441861
GTATCAA1800.014.251
ATCAACG2200.014.1136372
TCAACGC2300.013.53
CAACGCA2350.013.2127664
AACGCAG2550.012.1764715
TCTGCGT4900.011.29591827
TTGCTAC1452.1428496E-611.1724148
TGCGTTG2007.1431714E-910.79999927
AATGGGC900.00835989410.54
CAGAGTG3600.09.3759
AGAGTGA3600.09.37510
GCAGAGT3600.09.3758
ACGCAGA3700.09.1216216
Produced by FastQC (version 0.11.2)