FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_53.3520000005cfcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_53.3520000005cfcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18964
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG920.4851297194684666No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA720.37966673697532166No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC590.3111157983547775No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC480.2531111579835478No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA480.2531111579835478No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT420.22147226323560432No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA410.21619911411094708No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA390.20565281586163256No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA370.1951065176123181No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.1845602193630036No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA330.17401392111368907No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA310.1634676228643746No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA310.1634676228643746No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC290.1529213246150601No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA290.1529213246150601No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC260.13710187724108838No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGAAA250.13182872811643112No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA240.1265555789917739No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGCTC230.12128242986711665No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT230.12128242986711665No Hit
CCCTTAGACGTAGCTCACGTACTCTGCGTTGAT220.11600928074245939No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA200.1054629824931449No Hit
GTCCTAAGATGAGCTCAACGAGAACAGAAATCT200.1054629824931449No Hit
ACGTAGCTCAAAAAAAAAAAAAAAAAAAAAAAA200.1054629824931449No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT190.10018983336848766No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA190.10018983336848766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1207.2577677E-1015.7500011
ATCAACG1251.2951205E-915.122
GTTGATA657.1713026E-414.53846127
TCAACGC1353.8453436E-914.0000013
CGTTGAT1551.1823431E-1013.93548427
CAACGCA1451.5268961E-712.1034474
GCGTTGA2152.3156099E-79.41860526
AACGCAG1956.9328107E-69.05
TGCGTTG2801.0280866E-57.23214325
TCTGCGT4051.6400008E-86.999999527
CAGAGTG2551.9909763E-46.8823539
CTGCGTT3002.7137728E-56.750000524
ACGCAGA2602.527719E-46.751
GCAGAGT2602.527719E-46.758
AGAGTGA2653.1925944E-46.62264110
CGCAGAG2653.1925944E-46.6226417
GTGAATG4202.4690598E-76.42857171
TGAATGG4151.431199E-66.1807232
GAATGGG4651.5493024E-65.80645133
CTCTGCG4752.2654294E-65.68421126
Produced by FastQC (version 0.11.2)