FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_5.3520000005c9c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_5.3520000005c9c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13923
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG720.5171299288946347No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC530.38066508654743947No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA320.22983552395317103No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA260.18674136321195145No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC240.17237664296487826No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA220.15801192271780506No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG220.15801192271780506No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA210.1508295625942685No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT200.1436472024707319No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1436472024707319No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC180.12928248222365868No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC180.12928248222365868No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGCTC170.1221001221001221No Hit
ACGTAGCTCAAAAAAAAAAAAAAAAAAAAAAAA170.1221001221001221No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA160.11491776197658551No Hit
ACGCAGAGTGAATGGGTAGACGTAAAAAAAAAA160.11491776197658551No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA140.10055304172951231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT200.00832607927.01
TATCATC200.00832607927.02
TATCAAC404.692669E-420.251
TCAACGC550.00396765914.7272733
ATCAACG550.00396765914.7272732
AATGGGA1403.601599E-1014.4642864
CGTTGAT852.1630598E-514.29411827
CAACGCA600.007069643613.54
GCGTTGA900.00806744410.526
GTGAATG3350.010.4776121
TGAATGG3350.010.4776122
GAATGGG3600.010.1253
ACGCAGA1650.00104347338.1818181
CAGAGTG1650.00104347338.1818184
CGCAGAG1650.00104347338.1818182
TCTGCGT2501.8663741E-68.09999927
GCAGAGT1550.0055927987.83871033
AGAGTGA1750.00186798857.7142865
CTCTGCG2901.542378E-56.98275826
AGTGAAT2050.008763816.58536627
Produced by FastQC (version 0.11.2)