FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_46.3520000005ceda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_46.3520000005ceda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21663
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC860.3969902598901353No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA590.2723537829478835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.2677376171352075No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC360.1661819692563357No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA350.1615658034436597No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA250.11540414531689977No Hit
GTGAATGGGGAGTACGTTACTGCAAAAAAAAAA230.1061718136915478No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA220.10155564787887181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGT200.00842835827.021
CGTAGTC200.00842835827.022
GTCTGCG200.00842835827.026
TAGTCTG200.00842835827.024
AGTCTGC200.00842835827.025
GTAGTCT200.00842835827.023
TATCAAC756.384653E-1021.5999981
ATCAACG751.5405021E-819.82
TGCGTTG2400.019.68749827
TCAACGC701.6561353E-719.2857153
CGCCTCC603.9366653E-415.7499996
CAACGCA801.2839913E-515.18754
TCTGCGT8550.014.84210527
AACTCCT954.0394334E-614.2105267
GCCTCCT1252.2439053E-814.047
TTCTCCT750.002130602712.5999998
CTGCGTT3900.011.76923126
AACGCAG1051.6535069E-411.5714285
CTCTGCG10600.011.46226426
ACCTCCT1204.474649E-511.2499997
Produced by FastQC (version 0.11.2)