FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_41.3520000005ce3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_41.3520000005ce3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9062
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCGGTTGAACGTACTCTGCGTTGATACCA991.0924740675347604No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA390.43036857205914814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.2979474729640256No Hit
GAGTACGTTCAAAAAAAAAAAAAAAAAAAAAAA150.16552637386890312No Hit
GCCTATCGGTAAAAAAAAAAAAAAAAAAAAAAA140.15449128227764292No Hit
CCCTATCGGTTGAACGTACTCTGCGTTGATACC130.14345619068638268No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA130.14345619068638268No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA120.13242109909512248No Hit
CCCCTATCGGTTGAACGTACTCTGCGTTGATAC120.13242109909512248No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT110.12138600750386229No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA110.12138600750386229No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA100.11035091591260207No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.11035091591260207No Hit
CACCTATCGGTTGAACGTACTCTGCGTTGATAC100.11035091591260207No Hit
GCCTATCGGTTGAACGTACTCTGCGTTGATACC100.11035091591260207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT2550.020.11764727
GTCCTAT459.989523E-418.07
CTCTGCG3850.012.97402726
ACCTATC1357.146755E-712.08
TCCTATC1758.258212E-911.5714288
ACTCTGC4250.011.43529325
TACTCTG4300.010.98837224
TTGAACG5050.010.96039617
TGAACGT5050.010.96039618
GTTGAAC5100.010.85294116
GGTTGAA5100.010.85294115
TCGGTTG5100.010.85294113
ATCGGTT5100.010.85294112
CGGTTGA5100.010.85294114
GAACGTA5000.010.819
GTACTCT4600.010.56521823
AACGTAC5000.010.5320
TATCGGT5150.010.48543711
CGTACTC4650.010.45161322
ACGTACT4800.010.4062521
Produced by FastQC (version 0.11.2)