FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_38.3520000005cde8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_38.3520000005cde8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7921
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA1091.3760888776669613No Hit
TATCAACGCAGAGTGAATGGGCAACAACGTACT991.2498421916424693No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA831.0478474940032825No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA670.8458527963640954No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA570.7196061103396035No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA540.6817321045322561No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA480.605984092917561No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA460.5807347557126625No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA460.5807347557126625No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA460.5807347557126625No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA420.5302360813028658No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC420.5302360813028658No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG410.5176114127004166No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC400.5049867440979674No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC390.49236207549551825No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA370.4671127382906199No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA370.4671127382906199No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC360.45448806968817074No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA350.4418634010857215No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA350.4418634010857215No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA340.4292387324832723No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA320.4039893952783739No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA300.37874005807347555No Hit
GTGAATGGGCAACAACGTACTCTAAAAAAAAAA270.34086605226612804No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTAA260.32824138366367883No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.3156167150612297No Hit
CAACAACGTACTCTGCGAAAAAAAAAAAAAAAA250.3156167150612297No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA230.29036737785633127No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA230.29036737785633127No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTAAA230.29036737785633127No Hit
CAACGTACTCTGAAAAAAAAAAAAAAAAAAAAA220.2777427092538821No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA220.2777427092538821No Hit
ACGTACTCTGCGAAAAAAAAAAAAAAAAAAAAA210.2651180406514329No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCTG180.22724403484408537No Hit
GTGAATGGGCAACAACGTACTCTGCGAAAAAAA180.22724403484408537No Hit
ACGTACTCTGCGTAAAAAAAAAAAAAAAAAAAA180.22724403484408537No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGA170.21461936624163616No Hit
CAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA170.21461936624163616No Hit
GTGAATGGGCAACAACGTACTCTGCAAAAAAAA170.21461936624163616No Hit
GTGAATGGGCAACAACGTACTCTGAAAAAAAAA170.21461936624163616No Hit
GGCAACAACGTACTCAAAAAAAAAAAAAAAAAA170.21461936624163616No Hit
AACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA160.20199469763918695No Hit
CAACGTACTCTAAAAAAAAAAAAAAAAAAAAAA160.20199469763918695No Hit
CAACAACGTACTCTGCAAAAAAAAAAAAAAAAA150.18937002903673777No Hit
GTGAATGGGCAACAACGTACTCTGCGTAAAAAA150.18937002903673777No Hit
ACGTACTCTGAAAAAAAAAAAAAAAAAAAAAAA150.18937002903673777No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA150.18937002903673777No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1767453604342886No Hit
GTGAATGGGTGTTCGGCAAAAAAAAAAAAAAAA140.1767453604342886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1767453604342886No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1767453604342886No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1767453604342886No Hit
CTGTTCGGCAACAACGTACTCTGCGTTGATACC140.1767453604342886No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCA140.1767453604342886No Hit
GAGTGAATGGGCAACAACGTACTCTGCGAAAAA130.16412069183183942No Hit
CAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA120.15149602322939024No Hit
GAGTGAATGGGCAACAACGTACTCTGAAAAAAA120.15149602322939024No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA120.15149602322939024No Hit
TATCTACGCAGAGTGAATGGGCAACAACGTACT110.13887135462694106No Hit
CTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.13887135462694106No Hit
AACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA110.13887135462694106No Hit
GTGTTCGGCAAAAAAAAAAAAAAAAAAAAAAAA110.13887135462694106No Hit
GTGAATGGGTTGTTGCCAAAAAAAAAAAAAAAA100.12624668602449185No Hit
CAACAACGTACTCTGCGTAAAAAAAAAAAAAAA90.11362201742204268No Hit
TCAACGCAGAGTGAATGGGCAACAACGTACTCT90.11362201742204268No Hit
ACTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11362201742204268No Hit
ACGTACTCTAAAAAAAAAAAAAAAAAAAAAAAA90.11362201742204268No Hit
AACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA90.11362201742204268No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA80.10099734881959348No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA80.10099734881959348No Hit
GCAACAACGTACTCTAAAAAAAAAAAAAAAAAA80.10099734881959348No Hit
TCAACGCAGAGTGAATGGGCAACAACGTACTCA80.10099734881959348No Hit
GTACTCTGCGAAAAAAAAAAAAAAAAAAAAAAA80.10099734881959348No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCAA80.10099734881959348No Hit
GAGTGAATGGGCAACAACGTACTCTAAAAAAAA80.10099734881959348No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10099734881959348No Hit
CTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10099734881959348No Hit
GGCAACAACGTACTCTGCGAAAAAAAAAAAAAA80.10099734881959348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT1553.6379788E-1214.80645127
CTCTGCG2201.5588739E-910.43181826
GTGAATG2006.509963E-79.451
GAATGGG1903.7469217E-69.2368413
TGAATGG1903.7469217E-69.2368412
CGGCAAC2551.7858838E-89.013
TGTTCGG2653.3525794E-88.66037759
TTCGGCA2704.5489287E-88.511
GTTCGGC2704.5489287E-88.510
TCGGCAA2756.133996E-88.34545512
ACTCTGC3104.2564352E-77.403225425
TACTCTG3452.3441098E-66.65217424
GGCAACA3758.7106255E-66.1214
GCAACAA3801.0714242E-56.03947315
GTACTCT3901.6060341E-55.884615423
CAACAAC3901.6060341E-55.884615416
AACAACG3951.9575155E-55.810126317
ACAACGT4401.02879436E-45.21590918
CGTACTC4501.4477916E-45.122
CAACGTA4501.4477916E-45.119
Produced by FastQC (version 0.11.2)