FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_37.3520000005cdce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_37.3520000005cdce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12603
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA1601.2695389986511147No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.59509640561771No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA620.491946361977307No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA600.476077124494168No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA600.476077124494168No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC540.42846941204475125No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA320.253907799730223No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA320.253907799730223No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA270.21423470602237563No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA260.20630008728080618No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.1983654685392367No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA230.18249623105609777No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA210.16662699357295882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.15869237483138934No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC190.1507577560898199No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA160.1269538998651115No Hit
CTCTAGTACCAAAAAAAAAAAAAAAAAAAAAAA150.119019281123542No Hit
GTGAATGGGATCGGTACAAAAAAAAAAAAAAAA140.11108466238197254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC351.8878403E-423.1428591
TCTGCGT2050.018.43902627
ATCGGTA703.3678589E-617.3571438
ATCAACG500.002086230616.22
ACTCTGC2500.015.1225
CTCTGCG2450.014.87755126
GTACTCT2700.014.023
TACTCTG2700.014.024
TCAACGC600.00702070213.5000013
CAACGCA600.00702070213.5000014
ACGTACT2950.012.8135621
CGTACTC2950.012.8135622
AGACGTA3100.012.62903219
GACGTAC3000.012.59999920
GAGACGT3050.012.39344318
AGAGACG3200.011.812517
CGGTACT3350.011.68656810
GGTACTA3450.011.34782611
TCGGTAC3650.010.7260279
GCAGAGT900.00799589810.5000018
Produced by FastQC (version 0.11.2)