FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_32.3520000005cd11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_32.3520000005cd11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10140
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA1301.282051282051282No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA920.9072978303747534No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA760.7495069033530571No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAC740.7297830374753451No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA620.611439842209073No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA610.6015779092702169No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA570.5621301775147929No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA510.5029585798816568No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA500.4930966469428008No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA450.4437869822485207No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA400.39447731755424065No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA390.38461538461538464No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT380.37475345167652857No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA380.37475345167652857No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA370.36489151873767256No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC330.3254437869822485No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.3254437869822485No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA330.3254437869822485No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA320.3155818540433925No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA310.3057199211045365No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA310.3057199211045365No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.2859960552268245No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA280.27613412228796846No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA270.2662721893491124No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA270.2662721893491124No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA260.2564102564102564No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA250.2465483234714004No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA240.2366863905325444No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC230.22682445759368836No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA220.21696252465483234No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA220.21696252465483234No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA220.21696252465483234No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA210.20710059171597633No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA210.20710059171597633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.19723865877712032No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC200.19723865877712032No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA190.18737672583826429No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA190.18737672583826429No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG180.17751479289940827No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA180.17751479289940827No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA180.17751479289940827No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA180.17751479289940827No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA170.16765285996055226No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA170.16765285996055226No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA160.15779092702169625No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA160.15779092702169625No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA160.15779092702169625No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA150.14792899408284024No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.14792899408284024No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.14792899408284024No Hit
CTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.13806706114398423No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA140.13806706114398423No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA140.13806706114398423No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA130.1282051282051282No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA130.1282051282051282No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC130.1282051282051282No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA130.1282051282051282No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA120.1183431952662722No Hit
GAGTGAATGGGGCTAACTATAAAAAAAAAAAAA120.1183431952662722No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA120.1183431952662722No Hit
AACTATCCACGTACAAAAAAAAAAAAAAAAAAA120.1183431952662722No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA110.10848126232741617No Hit
GGGCTAACTATCCACGTACTCAAAAAAAAAAAA110.10848126232741617No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA110.10848126232741617No Hit
TATCTACGCAGAGTGAATGGGGCTAACTATCCA110.10848126232741617No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC110.10848126232741617No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA110.10848126232741617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG705.9026206E-921.21428727
GCAGAGT1003.5169796E-714.853
CGCAGAG1106.09798E-814.7272722
CTGCGTT1056.116261E-714.14285726
CAGAGTG1151.0523945E-714.0869574
AGAGTGA1201.772114E-713.55
ACGCAGA1252.9173862E-712.9599991
GGGGCTA955.840441E-512.7894747
ATGGGGC853.412795E-412.7058835
TGGGGCT853.412795E-412.7058836
TCTGCGT1901.6370905E-1012.07894727
AATGGGG958.715148E-411.3684224
GAGTGAA1604.5666275E-710.968756
AGTGAAT1604.5666275E-710.968757
GTGAATG2509.094947E-1210.7999991
TGAATGG2551.4551915E-1110.5882362
ATGGGCT1554.7496207E-59.5806455
GAATGGG2901.6189006E-109.3103443
TGGGCTA1606.7323796E-59.281256
AATGGGC1802.3442726E-59.04
Produced by FastQC (version 0.11.2)