FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_31.3520000005ccf6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_31.3520000005ccf6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10741
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA380.35378456382087325No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA370.344474443720324No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA350.3258542035192254No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA280.2606833628153803No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA280.2606833628153803No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA220.20482264221208454No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.20482264221208454No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA180.16758216180988733No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA180.16758216180988733No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA160.14896192160878877No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA150.13965180150823944No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA140.13034168140769015No Hit
CGTCAGACATCGCAAAAAAAAAAAAAAAAAAAA140.13034168140769015No Hit
ATGTCTGACGTACTCAAAAAAAAAAAAAAAAAA130.12103156130714085No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA130.12103156130714085No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA120.11172144120659157No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA120.11172144120659157No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA120.11172144120659157No Hit
CTTGCGATGTCTGACGTACTCTGCGTTGATACC110.10241132110604227No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA110.10241132110604227No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA110.10241132110604227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGGA1009.549694E-1017.554
TCTGCGT1355.086622E-813.00000127
GTGAATG2650.011.716981
TGAATGG2650.011.716982
CTCTGCG1553.0922274E-711.3225826
GAATGGG2800.011.0892863
ACTCTGC2008.198645E-68.77525
TACTCTG2101.5216874E-58.35714224
AATGGGG1550.00537820957.83870944
GTACTCT2356.252319E-57.46808523
GATGTCT2703.4830667E-46.500000513
ATGTCTG2703.4830667E-46.500000514
TGACGTA2951.3938108E-46.4067819
GCGATGT2754.3600978E-46.38181811
CGATGTC2754.3600978E-46.38181812
CTGACGT3001.7364134E-46.299999718
TGCGATG2805.432762E-46.26785710
ACGTACT3052.1542325E-46.196721621
TGTCTGA2856.739168E-46.157894615
CGTACTC2908.323768E-46.051724422
Produced by FastQC (version 0.11.2)