FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_29.3520000005cccf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_29.3520000005cccf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18124
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA1931.0648863385566099No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC1710.9435003310527478No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA1310.7227984992275436No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA510.2813948355771353No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.2813948355771353No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA500.2758772897815052No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA500.2758772897815052No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.2593246523946149No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA460.25380710659898476No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA440.24277201500772458No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA430.23725446921209448No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA420.23173692341646435No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA390.21518428602957407No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA380.20966674023394394No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA360.19863164864268373No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA340.18759655705142353No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.18207901125579343No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.17656146546016332No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA320.17656146546016332No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.160008828073273No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA290.160008828073273No Hit
GAGTGAATGGGGTCCAAAAAAAAAAAAAAAAAA290.160008828073273No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC280.15449128227764292No Hit
GCTTGGACCTTACGTACTCTGCGTTTATACCAC270.1489737364820128No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA270.1489737364820128No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA270.1489737364820128No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA260.14345619068638268No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA260.14345619068638268No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA260.14345619068638268No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.13242109909512248No Hit
TACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA220.12138600750386229No Hit
GTCCAAGCAAAAAAAAAAAAAAAAAAAAAAAAA210.11586846170823217No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA200.11035091591260207No Hit
GTGAATGGGGTCCAAAAAAAAAAAAAAAAAAAA200.11035091591260207No Hit
GAATGGGCCTTACGTACTCAAAAAAAAAAAAAA190.10483337011697197No Hit
GAGTGAATGGGGGTCCAAAAAAAAAAAAAAAAA190.10483337011697197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG200.00839228627.02
TCTACGC200.00839228627.03
TACGCAG200.00839228627.05
TATCTAC200.00839228627.01
CTACGCA200.00839228627.04
GTGAATA200.00839228627.01
TATCAAC707.026008E-515.4285721
GATACCA700.001252801213.527
TGATACC750.002107038912.59999926
CCTGCTT1101.795791E-512.2727268
TCTGCGT4900.012.12244927
TGCGTTG2056.002665E-1111.85365827
ACTGCTT800.003419729911.81257
GTTGATA800.003419729911.812524
TTGATAC800.003419729911.812525
ATCAACG959.3335606E-411.3684222
TTGCTTG1351.16688425E-511.08
CTGCGTT2501.6734703E-1010.2626
ACGCAGA2501.9099389E-99.721
CGCAGAG2501.9099389E-99.722
Produced by FastQC (version 0.11.2)