FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_28.3520000005cc99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_28.3520000005cc99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13682
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA1340.9793889782195586No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT710.5189299809969303No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA410.2996637918432978No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA350.25581055401257125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.2192661891536325No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA240.17541295132290602No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC210.15348633240754275No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA210.15348633240754275No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA190.13886858646396724No Hit
GTAGAGGACGAAAAAAAAAAAAAAAAAAAAAAA180.13155971349217951No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA180.13155971349217951No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA170.12425084052039176No Hit
GATCTCGTCCTCTACGTACTCTGCGTTGATACC160.116941967548604No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA150.10963309457681625No Hit
ACGCAGAGTGAATGGGATCTCGTCAAAAAAAAA140.1023242216050285No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT140.1023242216050285No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA140.1023242216050285No Hit
GATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAA140.1023242216050285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA401.6859643E-523.62527
TGATACC351.8992827E-423.14285926
TTGATAC351.8992827E-423.14285925
GTTGATA404.6866256E-420.2524
TATCAAC404.6866256E-420.251
GCGTTGA1252.00871E-814.0427
CGTTGAT600.007060939413.523
TCTGCGT3301.8189894E-129.40909127
TGCGTTG1802.5508962E-59.026
CTCTGCG3551.0913936E-118.74647826
ACTCTGC3804.7293724E-118.17105325
CTGCGTT2101.555393E-47.714285425
GTACTCT4256.002665E-117.623529423
TACTCTG4102.4556357E-107.573170724
CGTACTC4307.8216544E-117.534883522
ACGTACT4451.6916601E-107.280898621
CATCTCG2100.0013647117.07142838
TACGTAC4401.0986696E-97.056817520
CTACGTA4451.3951649E-96.97752819
TCTACGT4501.7644197E-96.899999618
Produced by FastQC (version 0.11.2)