FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_26.3520000005cc62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_26.3520000005cc62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12421
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1931.553820143305692No Hit
GACACAGACAGTACGTACTCTGCGTTGATACCA1841.4813622091619032No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT1431.1512760647290878No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA1381.1110216568714275No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA1220.982207551726914No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA1100.8855969728685291No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA830.668223170437163No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA770.6199178810079703No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA760.6118669994364383No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.5233073021495854No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA630.5072055390065212No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA630.5072055390065212No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA530.4266967232912004No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA520.4186458417196683No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA480.38644231543353996No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.3703405522904758No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA450.36228967071894375No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA410.3300861444328154No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.29788261814668704No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA370.29788261814668704No Hit
GACAGTACAAAAAAAAAAAAAAAAAAAAAAAAA360.289831736575155No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA360.289831736575155No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.2817808550036229No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA350.2817808550036229No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA340.2737299734320908No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA330.26567909186055877No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA320.25762821028902666No Hit
CAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA320.25762821028902666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.24152644714596247No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA290.2334755655744304No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA290.2334755655744304No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA280.2254246840028983No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA270.21737380243136623No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA250.2012720392883021No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA250.2012720392883021No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA250.2012720392883021No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA250.2012720392883021No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA240.19322115771676998No Hit
TATCTACGCAGAGTGAATGGGACACAGACAAAA240.19322115771676998No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA230.1851702761452379No Hit
GAGTGAATGGGACACAGACAGTAAAAAAAAAAA230.1851702761452379No Hit
ACGCAGAGTGAATGGGACACAGACAGTACAAAA230.1851702761452379No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA220.17711939457370582No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.17711939457370582No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.17711939457370582No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA210.16906851300217374No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGT210.16906851300217374No Hit
GGACACAGACAGTACAAAAAAAAAAAAAAAAAA200.16101763143064166No Hit
GAGTGAATGGGACACAGACAGTACAAAAAAAAA200.16101763143064166No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA200.16101763143064166No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA200.16101763143064166No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGA200.16101763143064166No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA200.16101763143064166No Hit
CAACTCAGAGTGAATGGGACACAGACAGAAAAA200.16101763143064166No Hit
GAATGGGGACACAGACAGAAAAAAAAAAAAAAA190.15296674985910957No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1449158682875775No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA180.1449158682875775No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA180.1449158682875775No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA170.1368649867160454No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGTA170.1368649867160454No Hit
GTGAATGGGACACAGACAGTACGTACTCAAAAA160.12881410514451333No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGAAA160.12881410514451333No Hit
GTGAATGGGACACAGACAGTACGAAAAAAAAAA160.12881410514451333No Hit
AATGGGACACAGACAAAAAAAAAAAAAAAAAAA160.12881410514451333No Hit
AGACAGTACGTACTCAAAAAAAAAAAAAAAAAA150.12076322357298123No Hit
GAGTGAATGGGGACACAGACAGAAAAAAAAAAA150.12076322357298123No Hit
ACGCAGAGTGAATGGGACACAGACAGTACGTAC150.12076322357298123No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC150.12076322357298123No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGAA150.12076322357298123No Hit
CAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.11271234200144915No Hit
ACGCAGAGTGAATGGGACACAAAAAAAAAAAAA140.11271234200144915No Hit
ATGGGACACAGACAAAAAAAAAAAAAAAAAAAA140.11271234200144915No Hit
ATGGGACACAGACAGAAAAAAAAAAAAAAAAAA140.11271234200144915No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA130.10466146042991707No Hit
AACGCAGAGTGAATGGGACACAGACAGTAAAAA130.10466146042991707No Hit
GAATGGGACACAGAAAAAAAAAAAAAAAAAAAA130.10466146042991707No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA130.10466146042991707No Hit
GGACACAGACAGAAAAAAAAAAAAAAAAAAAAA130.10466146042991707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTG405.1742427E-727.07692325
CACTGCT405.1742427E-727.07692327
CCACTGC405.1742427E-727.07692326
TACCACT405.1742427E-727.07692324
AACTCAG200.00834020626.9677432
ACTCAGA200.00834020626.9677433
TCAGAGT200.00834020626.9677435
CTCAGAG200.00834020626.9677434
ATACCAC451.4465495E-624.06837523
TATCAAC509.424647E-518.8774191
TTGATAC601.750411E-518.05128320
GTTGATA601.8134047E-517.97849519
GATACCA653.4843688E-516.66272222
TGATACC653.4843688E-516.66272221
ATCAACG700.001224790813.4838712
CGTTGAT905.7512755E-411.98566218
TCAACGC800.003345094411.7983883
CAACGCA800.003345094411.7983884
ACGCAGA1655.7179932E-811.4408611
CGCAGAG1751.2947567E-710.7870972
Produced by FastQC (version 0.11.2)