FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_25.3520000005cc49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_25.3520000005cc49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGAACCAAGACGTACTCTGCGTTGATACCA140.6862745098039216No Hit
AACCAAGACGTACTCAAAAAAAAAAAAAAAAAA120.5882352941176471No Hit
AACCAAGACAAAAAAAAAAAAAAAAAAAAAAAA110.5392156862745098No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA110.5392156862745098No Hit
GTGAATGGGCAACGAACCAAAAAAAAAAAAAAA110.5392156862745098No Hit
TATCAACGCAGAGTGAATGGGACAACGAACCAA90.4411764705882353No Hit
ACGCAGAGTGAATGGGCAACGAACAAAAAAAAA80.39215686274509803No Hit
GTGAATGGGACAACGAACCAAAAAAAAAAAAAA80.39215686274509803No Hit
AACCAAGACGAAAAAAAAAAAAAAAAAAAAAAA80.39215686274509803No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA60.29411764705882354No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.29411764705882354No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA60.29411764705882354No Hit
AACGCAGAGTGAATGGGCAACGAACCAAAAAAA60.29411764705882354No Hit
GTGAATGGGCAACGAACAAAAAAAAAAAAAAAA50.24509803921568626No Hit
ACGCAGAGTGAATGGGCAACGAACCAAAAAAAA50.24509803921568626No Hit
GAGTGAATGGGCAACGAACAAAAAAAAAAAAAA50.24509803921568626No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA50.24509803921568626No Hit
GTGAATGGGCCAACGAACCAAAAAAAAAAAAAA40.19607843137254902No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
CAACGAACCAAGACAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC40.19607843137254902No Hit
GTGAATGGGCAACGAACCAAGACGTACTCTGCG40.19607843137254902No Hit
CAACGAACCAAAAAAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
AACGCAGAGTGAATGGGCAACGAACAAAAAAAA40.19607843137254902No Hit
GCAACGAACCAAAAAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
GAGTGAATGGGCAACGAACCAAGACGAAAAAAA30.14705882352941177No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA30.14705882352941177No Hit
ACGCAGAGTGAATGGGCAACGAACCAAGACAAA30.14705882352941177No Hit
CAACGAACCAAGACGTAAAAAAAAAAAAAAAAA30.14705882352941177No Hit
GTGAATGGGCCAACGAAAAAAAAAAAAAAAAAA30.14705882352941177No Hit
ACGCAGAGTGAATGGGCCAACGAACCAAAAAAA30.14705882352941177No Hit
GTGAATGGGACAACGAACAAAAAAAAAAAAAAA30.14705882352941177No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA30.14705882352941177No Hit
TATCAACGCAGAGTGAATGGGCAACGAACCAAA30.14705882352941177No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA30.14705882352941177No Hit
AACCAAGACGTAAAAAAAAAAAAAAAAAAAAAA30.14705882352941177No Hit
CTGTCGCACCACGAGGCGCTGTCTACGAGTCGG30.14705882352941177No Hit
TATCAACGCAGAGTGAATGGGCAACGAACAAAA30.14705882352941177No Hit
GTGAATGGGACAACGAACCAAGACAAAAAAAAA30.14705882352941177No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA30.14705882352941177No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA30.14705882352941177No Hit
GTGAATGGGTCAACGAACCAAAAAAAAAAAAAA30.14705882352941177No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)