FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_24.3520000005cc2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_24.3520000005cc2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24547
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC1690.6884751700818837No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC1580.6436631767629445No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC650.264798142339186No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC640.26072432476473706No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC610.24850287204139No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC510.2077646962968998No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC510.2077646962968998No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC490.1996170611480018No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC410.16702652055240966No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC400.16295270297796066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.14258361510571557No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC300.1222145272334705No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA280.11406689208457246No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC280.11406689208457246No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT250.10184543936122542No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA250.10184543936122542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC356.244487E-627.027
GATACCA257.6100894E-426.99999826
TTGATAC1251.371518E-915.1227
TGATACC550.0040857214.72727327
ACTCCAC657.2806043E-414.5384617
GTTGATA1359.0867434E-712.00000126
TCTGCGT5150.011.27184527
TGAATGG1204.5318193E-511.252
CGTTGAT1607.00119E-610.12525
GAATGGG1351.4993545E-410.0000013
CTCTGCG5950.09.52941226
GTGAATG1300.00120013779.3461531
ACTCTGC6500.08.72307825
GCGTTGA1905.352049E-58.52631624
CTCCACA3602.055458E-108.258
CGTACTC7350.07.897958822
GTACTCT7200.07.87523
ACACGTA7550.07.867549419
CACGTAC7450.07.791946420
CACACGT7650.07.76470618
Produced by FastQC (version 0.11.2)