FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_17.3520000005cb3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_17.3520000005cb3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7860
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACGCACGTTACGTACTCTGCGTTGATACCA690.8778625954198473No Hit
CCATACGCACGTTACGTACTCTGCGTTGATACC240.3053435114503817No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA220.27989821882951654No Hit
ATACGCACGTTACGTACTCTGCGTTGATACCAC170.21628498727735368No Hit
ACATACGCACGTTACGTACTCTGCGTTGATACC170.21628498727735368No Hit
CCCATACGCACGTTACGTACTCTGCGTTGATAC160.2035623409669211No Hit
GCATACGCACGTTACGTACTCTGCGTTGATACC120.15267175572519084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10178117048346055No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10178117048346055No Hit
CTCATACGCACGTTACGTACTCTGCGTTGATAC80.10178117048346055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC300.002071902222.5000027
TCAAAAA350.005067919.28571512
TCTGCGT1101.5053769E-512.27272727
CTCTGCG1308.334481E-510.38461626
ACTCTGC1452.5202092E-49.31034425
ACGCACG1852.9509789E-58.75675712
CGCACGT1852.9509789E-58.75675713
GTACTCT1701.2074414E-48.73529423
TACTCTG1554.9275486E-48.70967724
CGTACTC1904.043975E-58.52631622
GCACGTT1751.6565926E-48.48571414
TACGCAC1955.4921104E-58.30769211
ATACGCA1955.4921104E-58.30769210
ACGTTAC1659.202408E-48.18181816
ACGTACT1853.0295615E-48.02702721
CGTTACG1700.00123826887.94117717
CACGTTA1700.00123826887.94117715
GTTACGT1700.00123826887.94117718
CATACGC2059.877411E-57.90243969
TTACGTA1750.00165042147.714285419
Produced by FastQC (version 0.11.2)