FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_14.3520000005caeb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_14.3520000005caeb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13022
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA2591.988941790815543No Hit
CAACAGAGCATACGTACTCTGCGTTGATACCAC1481.1365381661803102No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA690.5298725234219014No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA670.5145138995545999No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.39164490861618795No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA490.37628628474888653No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.33788972508063275No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA410.3148517892796805No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA400.3071724773460298No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA380.29181385347872835No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA360.2764552296114268No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA360.2764552296114268No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA360.2764552296114268No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA330.25341729381047456No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA320.24573798187682386No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA320.24573798187682386No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA300.23037935800952233No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.21502073414222087No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA280.21502073414222087No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA260.19966211027491937No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC250.1919827983412686No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC250.1919827983412686No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA240.18430348640761787No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA240.18430348640761787No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA240.18430348640761787No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA230.17662417447396714No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA210.16126555060666564No Hit
GTGAATGGGGCAACAGAAAAAAAAAAAAAAAAA210.16126555060666564No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA210.16126555060666564No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA210.16126555060666564No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1535862386730149No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT200.1535862386730149No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA190.14590692673936417No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA170.13054830287206268No Hit
GAGTGAATGGGCAACAGAGAAAAAAAAAAAAAA160.12286899093841193No Hit
CAACAGAGCATACGTAAAAAAAAAAAAAAAAAA160.12286899093841193No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC160.12286899093841193No Hit
GCCCGCAACAGAGCATACGTACTCTGCGTTGAT160.12286899093841193No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.12286899093841193No Hit
GCATACGTAAAAAAAAAAAAAAAAAAAAAAAAA150.11518967900476117No Hit
ACGCAGAGTGAATGGGCAACAGAAAAAAAAAAA150.11518967900476117No Hit
GGGCAACAGAGCATACGTACTCAAAAAAAAAAA150.11518967900476117No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT150.11518967900476117No Hit
CAACAGAGCATACGTACTCTGCGTTTATACCAC150.11518967900476117No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA140.10751036707111043No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA140.10751036707111043No Hit
CTGCAACAGAGCATACGTACTCTGCGTTGATAC140.10751036707111043No Hit
GAGCATACGTACTCTAAAAAAAAAAAAAAAAAA140.10751036707111043No Hit
ACGCAACAGAGCATACGTACTCTGCGTTGATAC140.10751036707111043No Hit
ACGCAGAGTGAATGGGGCAACAGAGAAAAAAAA140.10751036707111043No Hit
GTGAATGGGCAACAGAAAAAAAAAAAAAAAAAA140.10751036707111043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG200.00830639127.02
TCTACGC200.00830639127.03
TACGCAG257.417115E-427.05
TATCTAC200.00830639127.01
CTACGCA257.417115E-427.04
TCTGCGT2650.016.30188827
CTCTGCG3200.012.6562526
ACTCTGC3350.012.08955325
CGTTGAT800.003330423511.812527
TACTCTG3550.011.78873324
GTACTCT3750.011.1623
TGCGTTG900.00801938610.525
GCGTTGA900.00801938610.526
ATGGGCA1205.3727353E-410.12512
AGAGCAT4700.09.76595714
AATGGGC1300.00112151829.34615411
CGTACTC4500.09.29999922
CGCAACA1751.8055975E-59.2571438
ACGTACT4600.09.09782621
AGCATAC4800.09.016
Produced by FastQC (version 0.11.2)