FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_12.3520000005caa8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_12.3520000005caa8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15421
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.9143375915958757No Hit
GCGTAAGGATTCACGTACTCTGCGTTGATACCA1390.9013682640555086No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA1190.7716749886518384No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA1040.6744050320990856No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA820.5317424291550483No Hit
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA690.4474418001426626No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA660.427987808832112No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA650.4215031450619285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.3761104986706439No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA550.35665650736009336No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA540.3501718435899098No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA470.30477919719862523No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.2918098696582582No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA430.2788405421178912No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA380.2464172232669736No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA350.22696323195642307No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC350.22696323195642307No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA340.22047856818623954No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA340.22047856818623954No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.213993904416056No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA330.213993904416056No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA290.18805524933532194No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA290.18805524933532194No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA260.1686012580247714No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA250.1621165942545879No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC230.14914726671422088No Hit
GAATGGGCGTAAGGATAAAAAAAAAAAAAAAAA220.14266260294403735No Hit
GGCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAA200.12969327540367032No Hit
GAATGGGGCGTAAGGATAAAAAAAAAAAAAAAA200.12969327540367032No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA200.12969327540367032No Hit
GAATGGGCGTAAGGAAAAAAAAAAAAAAAAAAA190.1232086116334868No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGA180.1167239478633033No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1167239478633033No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA180.1167239478633033No Hit
GAGTGAATGGGGCGTAAGGAAAAAAAAAAAAAA180.1167239478633033No Hit
CACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA180.1167239478633033No Hit
GGCGTAAGGATTCACGTACTCTGCGTTGATACC180.1167239478633033No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA170.11023928409311977No Hit
ACGCAGAGTGAATGGGGCGTAAGGATAAAAAAA170.11023928409311977No Hit
GTGAATGGGGGCGTAAGGATAAAAAAAAAAAAA160.10375462032293625No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10375462032293625No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10375462032293625No Hit
ACGCAGAGTGAATGGGCGTAAGGATTCACGTAC160.10375462032293625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA200.00835382727.01
GTTGATA257.48079E-426.99999824
CGTTGAT257.48079E-426.99999823
GATACCA300.002165496822.527
TGATACC300.002165496822.526
TTGATAC300.002165496822.525
GCGTTGA300.002165496822.522
TATCAAC509.5307885E-518.91
TCTGCGT2900.017.22413627
TGCGTTG806.42498E-716.87527
ACGCGTA500.002115222216.1999997
CTGCGTT1001.0109473E-412.1526
TCAACGC800.003379596611.81253
ATCAACG800.003379596611.81252
CTCTGCG4350.011.79310326
ACTCTGC4950.010.90909125
AATGGGG1000.001412516710.7999994
ATGGGCG1401.6996148E-510.6071435
AATGGGC1401.6996148E-510.6071434
CAACGCA900.00813542410.54
Produced by FastQC (version 0.11.2)