FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_10.3520000005ca64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_10.3520000005ca64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7739
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA1121.4472154025067838No Hit
ATGCAGAGACGTACGTACTCTGCGTTGATACCA1101.4213722703191627No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA660.8528233621914976No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA550.7106861351595813No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA480.6202351725029074No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT380.4910195115648016No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA290.3747254167205065No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA280.36180385062669596No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA280.36180385062669596No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA260.3359607184390748No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA220.2842744540638325No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA220.2842744540638325No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA200.2584313218762114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.23258818968859024No Hit
ATGCAGAGACGTACGAAAAAAAAAAAAAAAAAA180.23258818968859024No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA180.23258818968859024No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.19382349140715854No Hit
ATGCAGAGACGTAAAAAAAAAAAAAAAAAAAAA150.19382349140715854No Hit
GTGAATGGGAATGCAGAGACAAAAAAAAAAAAA140.18090192531334798No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT130.1679803592195374No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1679803592195374No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA120.15505879312572685No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA120.15505879312572685No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA110.14213722703191625No Hit
GTGAATGGGATGCAGAGAAAAAAAAAAAAAAAA100.1292156609381057No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA100.1292156609381057No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA100.1292156609381057No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA90.11629409484429512No Hit
GCAGAGACGTACAAAAAAAAAAAAAAAAAAAAA90.11629409484429512No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11629409484429512No Hit
ATGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAA90.11629409484429512No Hit
GTGAATGGGATGCAGAGACGAAAAAAAAAAAAA90.11629409484429512No Hit
ATGCAGAGACGTACGTAAAAAAAAAAAAAAAAA80.10337252875048455No Hit
GTGAATGGGGAGACGTACGTACTCTAAAAAAAA80.10337252875048455No Hit
CCCATGCAGAGACGTACGTACTCTGCGTTGATA80.10337252875048455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC508.755596E-518.91
TCAACGC603.537376E-415.753
ATCAACG603.537376E-415.752
CAACGCA700.001140806713.54
AACGCAG1050.001918698810.2857141
GTGAATG2304.3199907E-68.2173911
TGAATGG2407.680594E-67.8752
GAATGGG2451.0140193E-57.7142863
Produced by FastQC (version 0.11.2)