FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate3_1.3520000005c94c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate3_1.3520000005c94c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA262.4074074074074074No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA232.1296296296296298No Hit
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA181.6666666666666667No Hit
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA181.6666666666666667No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA161.4814814814814816No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA141.2962962962962963No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA121.1111111111111112No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA111.0185185185185186No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA100.9259259259259258No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA90.8333333333333334No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA60.5555555555555556No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA60.5555555555555556No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA60.5555555555555556No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5555555555555556No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA50.4629629629629629No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA50.4629629629629629No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA50.4629629629629629No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA50.4629629629629629No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA50.4629629629629629No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.4629629629629629No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT40.3703703703703704No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA40.3703703703703704No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA40.3703703703703704No Hit
GAGTGAATGGGCAGACACACGTACTCTGCGTTG40.3703703703703704No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA40.3703703703703704No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA40.3703703703703704No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTG40.3703703703703704No Hit
GGCAGACACACGTACTCAAAAAAAAAAAAAAAA40.3703703703703704No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC40.3703703703703704No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
CACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA40.3703703703703704No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA40.3703703703703704No Hit
CCCATGAGCAGACACACGTACTCTGCGTTGATA30.2777777777777778No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
GCAGACACACGTACTCAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
ACGCAGAGAGAATGGGCAGACACAAAAAAAAAA30.2777777777777778No Hit
CCCCTATGAGCAGACACACGTACTCTGCGTTGA30.2777777777777778No Hit
AGACACACGTACTCAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA30.2777777777777778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA30.2777777777777778No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA30.2777777777777778No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
AACGCAGAGTGAATGGGCAGACACACGTAAAAA20.1851851851851852No Hit
CAACGCAGAGTGAATGGGCAGACACACAAAAAA20.1851851851851852No Hit
AACGCAGAGTGAATGGGCAGACACACAAAAAAA20.1851851851851852No Hit
CCCCTTCCATGAGCAGACACACGTACTCTGCGT20.1851851851851852No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGCAGACACACGTACAAAAAAAAAAA20.1851851851851852No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA20.1851851851851852No Hit
GAATGGGATGAGCAGACACACGTACTCAAAAAA20.1851851851851852No Hit
CCATGAGCAGACACACGTACTCTGCGTTGATAC20.1851851851851852No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GCCCCCATGAGCAGACACACGTACTCTGCGTTG20.1851851851851852No Hit
GAATGGGCAGACACACGTACTCTGCGTTGATAC20.1851851851851852No Hit
GAATGGGCAGACACACGTACTCTAAAAAAAAAA20.1851851851851852No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GATGAGCAGACACACGTACTCTGCGTTGATACC20.1851851851851852No Hit
CACATACTCAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GAGTGAATGGGCAGACACACGTAAAAAAAAAAA20.1851851851851852No Hit
ACACACGTACTCAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GAGTGAATGGGCAGACACACGAAAAAAAAAAAA20.1851851851851852No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACAA20.1851851851851852No Hit
GACACACGTACTCTAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CACGAACTCAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)