FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_96.3520000005c922.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_96.3520000005c922.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11702
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA2642.2560246111775766No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA1060.9058280635788754No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA1030.8801914202700394No Hit
ACGCAGAGTGAATGGGTTCCACGCAAAAAAAAA610.521278413946334No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA600.512732866176722No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA440.37600410186292943No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA380.3247308152452572No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA330.28200307639719707No Hit
AACGCAGAGTGAATGGGTTCCACGCAAAAAAAA310.26491198085797296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.2392753375491369No Hit
GTATCAACGCAGAGTGAATGGGTTCCACGCAAA280.2392753375491369No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA270.23072978977952485No Hit
GTGAATGGGTTCCACGCTAAAAAAAAAAAAAAA250.21363869424030082No Hit
GTTCCACGCTAAAAAAAAAAAAAAAAAAAAAAA240.20509314647068877No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA230.19654759870107674No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA220.18800205093146471No Hit
GTTCCACAAAAAAAAAAAAAAAAAAAAAAAAAA220.18800205093146471No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA210.17945650316185266No Hit
GAATGGGTTCCACGCAAAAAAAAAAAAAAAAAA200.17091095539224066No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA200.17091095539224066No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA190.1623654076226286No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC190.1623654076226286No Hit
ACGCAGAGTGAATGGGTTCCACGCTAAAAAAAA170.14527431208340455No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA170.14527431208340455No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCTAAA160.13672876431379252No Hit
GAGTGAATGGGTTCCACGCTAAAAAAAAAAAAA160.13672876431379252No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA160.13672876431379252No Hit
ACGCAGAGTGAATGGGTTCCACGAAAAAAAAAA150.1281832165441805No Hit
ATCAACGCAGAGTGAATGGGTTCCACGCAAAAA150.1281832165441805No Hit
GTGAATGGGGTTCCACGAAAAAAAAAAAAAAAA150.1281832165441805No Hit
GTGAATGGGTCCACGCTAAAAAAAAAAAAAAAA150.1281832165441805No Hit
TATCAACGCAGAGTGAATGGGTTCCACGAAAAA140.11963766877456845No Hit
CAACGCAGAGTGAATGGGTTCCACGCAAAAAAA130.11109212100495641No Hit
GTGAATGGGTTCCAAAAAAAAAAAAAAAAAAAA120.10254657323534438No Hit
GAGTGAATGGGTTCCAAAAAAAAAAAAAAAAAA120.10254657323534438No Hit
CGTTAAGCGTGAAAAAAAAAAAAAAAAAAAAAA120.10254657323534438No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA120.10254657323534438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA1305.456968E-1216.61538527
TATCAAC851.1504362E-615.8823521
ATCAACG953.6656984E-614.2105262
TCAACGC1006.2397976E-613.53
CAACGCA1151.7787515E-612.9130434
TGCGTTG1801.0495569E-912.026
CTGCGTT1953.7143764E-911.07692325
AATGGGG1102.3608914E-411.0454554
ACGCAGA2406.184564E-1110.68751
GCAGAGT2406.184564E-1110.68753
CGCAGAG2406.184564E-1110.68752
AGAGTGA2551.6370905E-1110.5882355
AACGCAG1452.3646522E-510.241385
CAGAGTG2653.8016879E-109.6792454
GAGTGAA3106.184564E-119.1451616
AGTGAAT3054.820322E-108.8524597
TCTGCGT2801.0844815E-88.67857227
GTGAATG4153.092282E-117.8072291
TGAATGG4153.092282E-117.8072292
CTCTGCG3352.2912354E-77.253731326
Produced by FastQC (version 0.11.2)