FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_95.3520000005c908.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_95.3520000005c908.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66733
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.3341675033341825No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1860.2787226709424123No Hit
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA1630.2442569643205011No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.23526591041913297No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1340.20080020379722174No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1250.18731362294516957No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1240.1858151139616082No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1070.16034046124106513No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1000.14985089835613563No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1000.14985089835613563No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.14685388038901293No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC920.13786282648764478No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA870.13037028156983801No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA850.12737326360271528No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA840.12587475461915396No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA780.1168837007177858No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.10339711986573359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA200.00856372226.9932441
CGCATAG200.00856372226.9932442
CTACTTC200.00856372226.9932444
CCCTACT200.00856372226.9932442
GTATCAT501.5220212E-724.2939191
CAACTCA451.6615886E-623.9939961
ACTCAGA451.6615886E-623.9939963
CTCAGAG451.6615886E-623.9939964
TCAGAGT401.823417E-523.619095
ATCTACG300.002245717422.494372
TCTACGC300.002245717422.494373
ACATTCC300.002245717422.494375
TACGCAG300.002245717422.494375
TATCTAC300.002245717422.494371
CTACGCA300.002245717422.494374
AACTCAG504.1495623E-621.5945952
TATCATC608.8162415E-720.2449322
TCATCGC651.8986066E-618.6876324
TGCGTTG6850.017.9432927
ATCATCG703.853156E-617.3527983
Produced by FastQC (version 0.11.2)