FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_94.3520000005c8ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_94.3520000005c8ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123626
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5800.4691569734521864No Hit
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC3720.3009075760762299No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA2400.1941339200491806No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA1800.14560044003688544No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC1750.1415559833691942No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1540.12456926536489087No Hit
CTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.11486256936243185No Hit
CCCCCCCTCCTTGCAGTAACGTACTCTGCGTTG1270.10272919935935808No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA1250.10111141669228156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT750.026.9987851
ATTAACG200.00858305626.9987853
TTAACGC200.00858305626.9987854
TTTATCT200.00858305626.9987853
CCCACAC352.0253504E-423.1418173
ATCTACG653.3251126E-922.8451252
TATCATC900.022.498992
CTCCTCT300.002253864222.4989874
TCTACGC658.3487976E-820.7682953
TGCGTTG13200.020.25728627
CAACTCA801.6407284E-920.249091
AACTCAG801.6407284E-920.249092
CTCAGAG801.6407284E-920.249094
AATCCTC608.92529E-720.2490885
TCAGAGT751.7302227E-819.7991095
TACGCAG701.8239734E-719.2848475
ATCATCG1057.2759576E-1219.2848473
CTACGCA701.8239734E-719.2848474
TCATCGC1057.2759576E-1219.2848474
CTCACCT350.005506974619.2848474
Produced by FastQC (version 0.11.2)