FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_91.3520000005c880.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_91.3520000005c880.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40086
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC4351.0851668911839545No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.7084767749338922No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA2240.5587985830464501No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA1570.39165793543880656No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1310.32679738562091504No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA1080.26942074539739563No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT950.23699047048844984No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA940.23449583395699244No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA870.21703337823679092No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA820.20456019557950406No Hit
ACGCAGAGTGAATGGGACATCCAGCAAAAAAAA760.18959237639075988No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA710.17711919373347304No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA680.16963528413910092No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA680.16963528413910092No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA670.16714064760764358No Hit
GTGAATGGGACATCCAGCTAAAAAAAAAAAAAA650.16215137454472883No Hit
CCCCCACATCCAGCTAGACGTACTCTGCGTTGA650.16215137454472883No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT630.1571621014818141No Hit
CCCCACATCCAGCTAGACGTACTCTGCGTTGAT620.15466746495035674No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA610.15217282841889937No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCTAG610.15217282841889937No Hit
ACGCAGAGTGAATGGGACATCCAGCTAGAAAAA580.14468891882452728No Hit
GAGTGAATGGGACATCCAGCAAAAAAAAAAAAA540.13471037269869782No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA520.12972109963578307No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCAAA490.12223719004141097No Hit
CATCCAGCTAGACGTACTCTGCGTTTATACCAC470.11724791697849622No Hit
CATCCAGCTAGAAAAAAAAAAAAAAAAAAAAAA410.10228009778975203No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA410.10228009778975203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.00852863626.9900131
ATACCAC906.5992936E-918.01583527
TGACATC751.3327794E-414.3946727
AATGGGT802.3055002E-413.4950074
GATACCA1202.3042594E-713.49500626
TGATACC1202.3042594E-713.49500625
TCTGCGT12450.013.34908227
TTGATAC1306.0051025E-712.472524
GCGTTGA3750.011.53013327
TATCAAC2304.7293724E-1111.1480481
CTCTGCG15700.010.57252126
CGACATC1701.3413555E-610.3197117
GTTGATA1607.2961684E-610.13390623
ATGGGGA1351.5608728E-49.9963015
ACTCTGC17200.09.72895825
TCACATC1402.252369E-49.6392917
TACTCTG17750.09.36315824
GTATCAA2051.4409652E-69.2161021
TCAACGC2954.3837645E-109.1491583
CGTACTC18500.09.12964422
Produced by FastQC (version 0.11.2)