FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_90.3520000005c85a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_90.3520000005c85a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60132
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA3040.5055544468835229No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC2840.4722942859043438No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA2730.4540011973657953No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA2340.38914388345639594No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG2100.34923169028138096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.3209605534490787No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA1850.307656489057407No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA1720.28603738442094057No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA1560.2594292556375973No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA1550.2577662475886383No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA1530.2544402314907204No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA1450.24113616709904875No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG1350.2245060866094592No Hit
GGAACGTACTCTGCGTTGATACAAAAAAAAAAA1230.2045499900219517No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.19623494977715691No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.17960486928756736No Hit
GTATCAACGCAGAGTGAATGGGAAACGTACTCT990.16463779684693672No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.16131178074901883No Hit
GAACGTACTCTGCGTTGATACAAAAAAAAAAAA970.16131178074901883No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.15964877270005987No Hit
GTGAATGGGAACGTACTCTGCGTTGATACCAAA960.15964877270005987No Hit
AACGCAGAGTGAATGGGAACGTACTCTGCGTTG960.15964877270005987No Hit
GAAACGTACTCTGCGTTGATACAAAAAAAAAAA940.15632275660214195No Hit
GAAACGTACTCTGCGAAAAAAAAAAAAAAAAAA900.14967072440630613No Hit
AACGTACTCTGCGTTGAAAAAAAAAAAAAAAAA890.14800771635734716No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA890.14800771635734716No Hit
GGAACGTACTCTGCGTTGATACCAAAAAAAAAA840.1396926761125524No Hit
GTACTCTGCGTTGATACAAAAAAAAAAAAAAAA840.1396926761125524No Hit
GCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.1396926761125524No Hit
GGTATCAACGCAGAGTGAATGGGAACGTACTCT830.13802966806359343No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.12971462781879864No Hit
GTGAATGGGAAACGTACTCTGCGTAAAAAAAAA780.12971462781879864No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA760.12638861172088073No Hit
AACGTACTCTGCGTTAAAAAAAAAAAAAAAAAA740.12306259562296282No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA740.12306259562296282No Hit
GATACCAAAAAAAAAAAAAAAAAAAAAAAAAAA730.12139958757400385No Hit
ACTCTGCGTTGATACCAAAAAAAAAAAAAAAAA700.116410563427127No Hit
GAACGTACTCTGCGTTGATACCAAAAAAAAAAA680.11308454732920907No Hit
GAGTGAATGGGAACGTACTCTGCGTTGATACAA670.11142153928025013No Hit
GTATCATCGCAGAGTGAATGGGAACGTACTCTG660.10975853123129115No Hit
GATACAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1080955231823322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG405.881975E-727.022
ACGCTGA405.881975E-727.021
ATCTACG604.3655746E-1127.022
GTATCAT307.01243E-527.021
TATCTAC653.6379788E-1227.021
TATCATC307.01243E-527.022
CTGAGTG405.925067E-726.9975054
GCTGAGT405.925067E-726.9975053
TGAGTGA405.925067E-726.9975055
TCTACGC554.674803E-1026.9975013
CTACGCA601.2823875E-924.7477114
CAACTCA401.8056013E-523.64251
TCAGAGT401.817312E-523.6228165
TACGCAG653.2414391E-922.844045
GTTCAGT404.982657E-420.2312836
CGTAGAG350.005476213519.283933
ACTCAGA501.0148505E-418.8982523
CTCAGAG501.0148505E-418.8982524
AGAATGG807.256076E-716.8734414
ATCATCG500.002215777316.1985023
Produced by FastQC (version 0.11.2)