FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_9.3520000005be3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_9.3520000005be3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71235
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC5320.7468238927493508No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC2640.3706043377553169No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA2540.35656629465852463No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA2510.3523548817294869No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC2000.28076086193584615No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1970.27654944900680845No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1930.27093423176809156No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1760.24706955850354462No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA1580.22180108092931844No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA1190.16705271285182846No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA1120.15722608268407384No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1100.15441847406471537No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1080.15161086544535693No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA990.13897662665824384No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT950.1333614094195269No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA950.1333614094195269No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC930.13055380080016846No Hit
CCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA920.12914999649048922No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA900.12634238787113075No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA890.12493858356145153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.12213097494209307No Hit
ACTACGTACCGTAAAAAAAAAAAAAAAAAAAAA830.11651575770337615No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA820.11511195339369693No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA770.10809293184530076No Hit
TCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA740.10388151891626307No Hit
GTGAATGGGCTACGTACAAAAAAAAAAAAAAAA740.10388151891626307No Hit
ACGCAGAGTGAATGGGCTACGTACCGAAAAAAA730.10247771460658384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG405.94624E-727.02
AACGTAG307.065982E-527.01
CTGAGTG405.94624E-727.04
ACGCTGA405.94624E-727.01
GTATCAT405.94624E-727.01
TATCATC405.94624E-727.02
GCTGAGT405.94624E-727.03
TGAGTGA405.94624E-727.05
TCGCAGA504.1471085E-621.5999987
ATCGCAG504.1471085E-621.5999986
TCATCGC504.1471085E-621.5999984
TGATACC2350.021.2553227
GCAGAGA404.9639965E-420.258
ATCATCG559.461133E-619.6363623
CATCGCA559.461133E-619.6363625
TAGCTAC350.00548302719.2857156
TCTACGC350.00548302719.2857153
CAGAGAG450.001096361218.0000029
AGAGAGA450.001096361218.00000210
ACTCAGA602.0031255E-518.03
Produced by FastQC (version 0.11.2)